Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project

Peter Williams; David Schieltz; Randall W. Nelson; Chou Wen Chou; Cong Wen Luo; Robert Thomas

doi:10.1117/12.146708

Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project

Peter Williams, David Schieltz, Randall W. Nelson, Chou Wen Chou, Cong Wen Luo, Robert Thomas

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Conference article › peer-review

3 Scopus citations

Abstract

Techniques have been developed to volatilize intact massive DNA molecules using pulsed laser ablation of thin frozen films of aqueous DNA solutions. Electrophoresis assay of the ablated DNA shows that molecules as massive as ∼400,000 Da can be ablated intact. It has been possible to obtain time-of-flight mass spectra of ablated multicomponent mixtures of single-stranded DNA with masses up to 18,000 Da (a 60-nucleotide DNA oligomer). The possible application of time-of-flight mass spectrometry to the analysis and readout of DNA sequence mixtures, and the potential thereby to accelerate the Human Genome project, are discussed.

Original language	English (US)
Pages (from-to)	86-93
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1891
DOIs	https://doi.org/10.1117/12.146708
State	Published - Jun 24 1993
Event	Advances in DNA Sequencing Technology 1993 - Los Angeles, United States Duration: Jan 17 1993 → Jan 22 1993

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.146708

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Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project. / Williams, Peter; Schieltz, David; Nelson, Randall W. et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1891, 24.06.1993, p. 86-93.

Research output: Contribution to journal › Conference article › peer-review

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title = "Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project",

abstract = "Techniques have been developed to volatilize intact massive DNA molecules using pulsed laser ablation of thin frozen films of aqueous DNA solutions. Electrophoresis assay of the ablated DNA shows that molecules as massive as ∼400,000 Da can be ablated intact. It has been possible to obtain time-of-flight mass spectra of ablated multicomponent mixtures of single-stranded DNA with masses up to 18,000 Da (a 60-nucleotide DNA oligomer). The possible application of time-of-flight mass spectrometry to the analysis and readout of DNA sequence mixtures, and the potential thereby to accelerate the Human Genome project, are discussed.",

author = "Peter Williams and David Schieltz and Nelson, {Randall W.} and Chou, {Chou Wen} and Luo, {Cong Wen} and Robert Thomas",

note = "Funding Information: This work is supported by Department of Energy Grant DE-FGO2-91ER61 127. Publisher Copyright: {\textcopyright} 1993 SPIE. All rights reserved.; Advances in DNA Sequencing Technology 1993 ; Conference date: 17-01-1993 Through 22-01-1993",

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AU - Luo, Cong Wen

AU - Thomas, Robert

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AB - Techniques have been developed to volatilize intact massive DNA molecules using pulsed laser ablation of thin frozen films of aqueous DNA solutions. Electrophoresis assay of the ablated DNA shows that molecules as massive as ∼400,000 Da can be ablated intact. It has been possible to obtain time-of-flight mass spectra of ablated multicomponent mixtures of single-stranded DNA with masses up to 18,000 Da (a 60-nucleotide DNA oligomer). The possible application of time-of-flight mass spectrometry to the analysis and readout of DNA sequence mixtures, and the potential thereby to accelerate the Human Genome project, are discussed.

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Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project

Abstract

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