Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project

Peter Williams, David Schieltz, Randall Nelson, Chou Wen Chou, Cong Wen Luo, Robert Thomas

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

Techniques have been developed to volatilize intact massive DNA molecules using pulsed laser ablation of thin frozen films of aqueous DNA solutions. Electrophoresis assay of the ablated DNA shows that molecules as massive as ∼400,000 Da can be ablated intact. It has been possible to obtain time-of-flight mass spectra of ablated multicomponent mixtures of single-stranded DNA with masses up to 18,000 Da (a 60-nucleotide DNA oligomer). The possible application of time-of-flight mass spectrometry to the analysis and readout of DNA sequence mixtures, and the potential thereby to accelerate the Human Genome project, are discussed.

Original languageEnglish (US)
Pages (from-to)86-93
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1891
DOIs
StatePublished - Jun 24 1993
EventAdvances in DNA Sequencing Technology 1993 - Los Angeles, United States
Duration: Jan 17 1993Jan 22 1993

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: Applications to the human genome project'. Together they form a unique fingerprint.

  • Cite this