Time of flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Time of flight mass spectrometry offers an extremely rapid and accurate alternative to gel electrophoresis for sizing DNA fragments in the Sanger sequencing process, if large single-stranded DNA molecules can be volatilized and ionized without fragmentation. A process based on pulsed laser ablation of thin frozen films of DNA solutions has been shown to ablate intact DNA molecules up to ≈400 kDa in mass, and also has been shown to yield molecular ions of single-stranded DNA up to ≈18 500 Da. The theoretical basis and the progress to date in this approach are described and the potential impact of mass spectrometry on large-scale DNA sequencing is discussed.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalInternational Journal of Mass Spectrometry and Ion Processes
Volume131
Issue numberC
DOIs
StatePublished - Feb 24 1994

Fingerprint

genome
Mass spectrometry
DNA
mass spectroscopy
deoxyribonucleic acid
Genes
aqueous solutions
Lasers
Single-Stranded DNA
lasers
sequencing
Molecules
Laser ablation
Electrophoresis
Pulsed lasers
Gels
sizing
Ions
electrophoresis
molecular ions

Keywords

  • DNA
  • Laser ablation
  • Sequencing

ASJC Scopus subject areas

  • Spectroscopy

Cite this

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AB - Time of flight mass spectrometry offers an extremely rapid and accurate alternative to gel electrophoresis for sizing DNA fragments in the Sanger sequencing process, if large single-stranded DNA molecules can be volatilized and ionized without fragmentation. A process based on pulsed laser ablation of thin frozen films of DNA solutions has been shown to ablate intact DNA molecules up to ≈400 kDa in mass, and also has been shown to yield molecular ions of single-stranded DNA up to ≈18 500 Da. The theoretical basis and the progress to date in this approach are described and the potential impact of mass spectrometry on large-scale DNA sequencing is discussed.

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