Time of flight mass spectrometry offers an extremely rapid and accurate alternative to gel electrophoresis for sizing DNA fragments in the Sanger sequencing process, if large single-stranded DNA molecules can be volatilized and ionized without fragmentation. A process based on pulsed laser ablation of thin frozen films of DNA solutions has been shown to ablate intact DNA molecules up to ≈400 kDa in mass, and also has been shown to yield molecular ions of single-stranded DNA up to ≈18 500 Da. The theoretical basis and the progress to date in this approach are described and the potential impact of mass spectrometry on large-scale DNA sequencing is discussed.
|Original language||English (US)|
|Number of pages||10|
|Journal||International Journal of Mass Spectrometry and Ion Processes|
|State||Published - Feb 24 1994|
- Laser ablation
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