Abstract
Time of flight mass spectrometry offers an extremely rapid and accurate alternative to gel electrophoresis for sizing DNA fragments in the Sanger sequencing process, if large single-stranded DNA molecules can be volatilized and ionized without fragmentation. A process based on pulsed laser ablation of thin frozen films of DNA solutions has been shown to ablate intact DNA molecules up to ≈400 kDa in mass, and also has been shown to yield molecular ions of single-stranded DNA up to ≈18 500 Da. The theoretical basis and the progress to date in this approach are described and the potential impact of mass spectrometry on large-scale DNA sequencing is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 335-344 |
| Number of pages | 10 |
| Journal | International Journal of Mass Spectrometry and Ion Processes |
| Volume | 131 |
| Issue number | C |
| DOIs | |
| State | Published - Feb 24 1994 |
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Keywords
- DNA
- Laser ablation
- Sequencing
ASJC Scopus subject areas
- Spectroscopy
Cite this
Time of flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions : applications to the Human Genome Project. / Williams, Peter.
In: International Journal of Mass Spectrometry and Ion Processes, Vol. 131, No. C, 24.02.1994, p. 335-344.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time of flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions
T2 - applications to the Human Genome Project
AU - Williams, Peter
PY - 1994/2/24
Y1 - 1994/2/24
N2 - Time of flight mass spectrometry offers an extremely rapid and accurate alternative to gel electrophoresis for sizing DNA fragments in the Sanger sequencing process, if large single-stranded DNA molecules can be volatilized and ionized without fragmentation. A process based on pulsed laser ablation of thin frozen films of DNA solutions has been shown to ablate intact DNA molecules up to ≈400 kDa in mass, and also has been shown to yield molecular ions of single-stranded DNA up to ≈18 500 Da. The theoretical basis and the progress to date in this approach are described and the potential impact of mass spectrometry on large-scale DNA sequencing is discussed.
AB - Time of flight mass spectrometry offers an extremely rapid and accurate alternative to gel electrophoresis for sizing DNA fragments in the Sanger sequencing process, if large single-stranded DNA molecules can be volatilized and ionized without fragmentation. A process based on pulsed laser ablation of thin frozen films of DNA solutions has been shown to ablate intact DNA molecules up to ≈400 kDa in mass, and also has been shown to yield molecular ions of single-stranded DNA up to ≈18 500 Da. The theoretical basis and the progress to date in this approach are described and the potential impact of mass spectrometry on large-scale DNA sequencing is discussed.
KW - DNA
KW - Laser ablation
KW - Sequencing
UR - http://www.scopus.com/inward/record.url?scp=0013376866&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0013376866&partnerID=8YFLogxK
U2 - 10.1016/0168-1176(93)03887-R
DO - 10.1016/0168-1176(93)03887-R
M3 - Article
AN - SCOPUS:0013376866
VL - 131
SP - 335
EP - 344
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
SN - 1387-3806
IS - C
ER -