Abstract
We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.
| Original language | English (US) |
|---|---|
| Title of host publication | MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest |
| Pages | 887-890 |
| Number of pages | 4 |
| DOIs | |
| State | Published - Jun 1 2010 |
| Event | 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: Jan 24 2010 → Jan 28 2010 |
Publication series
| Name | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| ISSN (Print) | 1084-6999 |
Other
| Other | 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 |
|---|---|
| Country | China |
| City | Hong Kong |
| Period | 1/24/10 → 1/28/10 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
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Cite this
Choi, S., & Chae, J. (2010). Thyroglobulin detection using competitive protein adsorption. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 887-890). [5442349] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442349