Thyroglobulin detection using competitive protein adsorption

Seokheun Choi; Junseok Chae

doi:10.1109/MEMSYS.2010.5442349

Thyroglobulin detection using competitive protein adsorption

Seokheun Choi, Junseok Chae

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.

Original language	English (US)
Title of host publication	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages	887-890
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442349
State	Published - Jun 1 2010
Event	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: Jan 24 2010 → Jan 28 2010

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Country/Territory	China
City	Hong Kong
Period	1/24/10 → 1/28/10

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/MEMSYS.2010.5442349

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Thyroglobulin detection using competitive protein adsorption. / Choi, Seokheun; Chae, Junseok.

MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 887-890 5442349 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Choi, S & Chae, J 2010, Thyroglobulin detection using competitive protein adsorption. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442349, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 887-890, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 1/24/10. https://doi.org/10.1109/MEMSYS.2010.5442349

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title = "Thyroglobulin detection using competitive protein adsorption",

abstract = "We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.",

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AB - We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.

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Thyroglobulin detection using competitive protein adsorption

Abstract

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Other

ASJC Scopus subject areas

UN SDGs

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