Abstract

We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages887-890
Number of pages4
DOIs
StatePublished - 2010
Event23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China
Duration: Jan 24 2010Jan 28 2010

Other

Other23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
CountryChina
CityHong Kong
Period1/24/101/28/10

Fingerprint

Thyroglobulin
proteins
Proteins
Adsorption
adsorption
Surface plasmon resonance
surface plasmon resonance
affinity
fibrinogen
biomarkers
Immunoglobulin G
albumins
Microfluidics
quantitative analysis
Haptoglobins
cancer
Tumor Biomarkers
Fibrinogen
Albumins
Chemical analysis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Choi, S., & Chae, J. (2010). Thyroglobulin detection using competitive protein adsorption. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 887-890). [5442349] https://doi.org/10.1109/MEMSYS.2010.5442349

Thyroglobulin detection using competitive protein adsorption. / Choi, Seokheun; Chae, Junseok.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. p. 887-890 5442349.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Choi, S & Chae, J 2010, Thyroglobulin detection using competitive protein adsorption. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 5442349, pp. 887-890, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 1/24/10. https://doi.org/10.1109/MEMSYS.2010.5442349
Choi S, Chae J. Thyroglobulin detection using competitive protein adsorption. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. p. 887-890. 5442349 https://doi.org/10.1109/MEMSYS.2010.5442349
Choi, Seokheun ; Chae, Junseok. / Thyroglobulin detection using competitive protein adsorption. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. pp. 887-890
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