TY - GEN
T1 - Thyroglobulin detection using competitive protein adsorption
AU - Choi, Seokheun
AU - Chae, Junseok
PY - 2010/6/1
Y1 - 2010/6/1
N2 - We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.
AB - We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.
UR - http://www.scopus.com/inward/record.url?scp=77952758720&partnerID=8YFLogxK
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U2 - 10.1109/MEMSYS.2010.5442349
DO - 10.1109/MEMSYS.2010.5442349
M3 - Conference contribution
AN - SCOPUS:77952758720
SN - 9781424457649
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 887
EP - 890
BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Y2 - 24 January 2010 through 28 January 2010
ER -