Abstract
We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
| Pages | 887-890 |
| Number of pages | 4 |
| DOIs | |
| State | Published - 2010 |
| Event | 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: Jan 24 2010 → Jan 28 2010 |
Other
| Other | 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 |
|---|---|
| Country | China |
| City | Hong Kong |
| Period | 1/24/10 → 1/28/10 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Mechanical Engineering
- Condensed Matter Physics
- Electronic, Optical and Magnetic Materials
Cite this
Thyroglobulin detection using competitive protein adsorption. / Choi, Seokheun; Chae, Junseok.
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. p. 887-890 5442349.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Thyroglobulin detection using competitive protein adsorption
AU - Choi, Seokheun
AU - Chae, Junseok
PY - 2010
Y1 - 2010
N2 - We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.
AB - We report a unique sensing mechanism based on competitive proteins' adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.
UR - http://www.scopus.com/inward/record.url?scp=77952758720&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952758720&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2010.5442349
DO - 10.1109/MEMSYS.2010.5442349
M3 - Conference contribution
AN - SCOPUS:77952758720
SN - 9781424457649
SP - 887
EP - 890
BT - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ER -