Three phase countercurrent extraction

Yu Fu Liu; Antonio Garcia

doi:10.1080/00986440008912836

Three phase countercurrent extraction

Yu Fu Liu, Antonio Garcia

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Partitioning of bovine serum albumin (BSA) in an aqueous double countercurrent distribution (DCCD) using a three-phase system was studied and compared with countercurrent distribution using a two-phase system. The two-phase system was composed of Dextran and Polyethylene glycol (PEG) solutions, and the three-phase system consisted of Dextran, Ficoll, and PEG solutions. In the double countercurrent distribution experiment, BSA was partitioned from the stationary bottom phase into the moving middle and top phases. Two peaks resulted from these transfers. A mathematical model that predicts peak positions and shapes based on the partitioning behavior of the solute is provided. Based on model predictions, the DCCD method has potential extraction selectivity and recovery advantages over the traditional CCD method.

Original language	English (US)
Pages (from-to)	239-259
Number of pages	21
Journal	Chemical Engineering Communications
Volume	182
DOIs	https://doi.org/10.1080/00986440008912836
State	Published - 2000

Keywords

Aqueous phase
Bovine serum albumin
Extraction
Protein separation
Three phase

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1080/00986440008912836

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title = "Three phase countercurrent extraction",

abstract = "Partitioning of bovine serum albumin (BSA) in an aqueous double countercurrent distribution (DCCD) using a three-phase system was studied and compared with countercurrent distribution using a two-phase system. The two-phase system was composed of Dextran and Polyethylene glycol (PEG) solutions, and the three-phase system consisted of Dextran, Ficoll, and PEG solutions. In the double countercurrent distribution experiment, BSA was partitioned from the stationary bottom phase into the moving middle and top phases. Two peaks resulted from these transfers. A mathematical model that predicts peak positions and shapes based on the partitioning behavior of the solute is provided. Based on model predictions, the DCCD method has potential extraction selectivity and recovery advantages over the traditional CCD method.",

keywords = "Aqueous phase, Bovine serum albumin, Extraction, Protein separation, Three phase",

author = "Liu, {Yu Fu} and Antonio Garcia",

year = "2000",

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language = "English (US)",

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T1 - Three phase countercurrent extraction

AU - Liu, Yu Fu

AU - Garcia, Antonio

PY - 2000

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N2 - Partitioning of bovine serum albumin (BSA) in an aqueous double countercurrent distribution (DCCD) using a three-phase system was studied and compared with countercurrent distribution using a two-phase system. The two-phase system was composed of Dextran and Polyethylene glycol (PEG) solutions, and the three-phase system consisted of Dextran, Ficoll, and PEG solutions. In the double countercurrent distribution experiment, BSA was partitioned from the stationary bottom phase into the moving middle and top phases. Two peaks resulted from these transfers. A mathematical model that predicts peak positions and shapes based on the partitioning behavior of the solute is provided. Based on model predictions, the DCCD method has potential extraction selectivity and recovery advantages over the traditional CCD method.

AB - Partitioning of bovine serum albumin (BSA) in an aqueous double countercurrent distribution (DCCD) using a three-phase system was studied and compared with countercurrent distribution using a two-phase system. The two-phase system was composed of Dextran and Polyethylene glycol (PEG) solutions, and the three-phase system consisted of Dextran, Ficoll, and PEG solutions. In the double countercurrent distribution experiment, BSA was partitioned from the stationary bottom phase into the moving middle and top phases. Two peaks resulted from these transfers. A mathematical model that predicts peak positions and shapes based on the partitioning behavior of the solute is provided. Based on model predictions, the DCCD method has potential extraction selectivity and recovery advantages over the traditional CCD method.

KW - Aqueous phase

KW - Bovine serum albumin

KW - Extraction

KW - Protein separation

KW - Three phase

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JO - Chemical Engineering Communications

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Three phase countercurrent extraction

Abstract

Keywords

ASJC Scopus subject areas

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