Abstract

We present a diagnostic platform for measuring three-dimensional three-component (3D3C) velocity fields in microscopic volumes. The imaging system uses high-speed Nipkow spinning disk confocal microscopy. Confocal microscopy provides optical sectioning using pinhole spatial filtering which rejects light originating from out-of-focus objects. The system accomplishes volumetric scanning by rapid translation of the high numerical aperture objective using a piezo objective positioner. The motion of fluorescent microspheres is quantified using 3D3C super resolution particle-imaging velocimetry with instantaneous spatial resolutions of the order of 5m or less in all three dimensions. We examine 3D3C flow in a PDMS microchannel with an expanding section at 3D acquisition rates of 30Hz, and find strong agreement with a computational model. Equations from the PIV and PTV literature adapted for a scanning objective provide estimates of maximum measurable velocity. The technique allows for isosurface visualization of 3D particle motion and robust high spatial resolution velocity measurements without requiring a calibration step or reconstruction algorithms.

Original languageEnglish (US)
Article number085304
JournalMeasurement Science and Technology
Volume23
Issue number8
DOIs
StatePublished - Aug 2012

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Confocal Microscopy
Confocal microscopy
Spatial Resolution
microbalances
Velocity measurement
Scanning
spatial resolution
microscopy
Isosurface
Spatial Filtering
Three-dimensional
Velocity Measurement
spatial filtering
Microspheres
scanning
Motion
Super-resolution
Microchannel
particle motion
Reconstruction Algorithm

Keywords

  • confocal
  • micro PIV
  • microfluidics
  • microscopy
  • objective positioner
  • particle-image velocimetry
  • particle-tracking velocimetry
  • piezo
  • piezo actuated
  • PIV
  • PTV
  • super resolution PIV
  • temporally resolved
  • time resolved
  • volumetric scanning

ASJC Scopus subject areas

  • Applied Mathematics
  • Instrumentation

Cite this

Three-dimensional three-component particle velocimetry for microscale flows using volumetric scanning. / Klein, S. A.; Moran, J. L.; Frakes, David; Posner, J. D.

In: Measurement Science and Technology, Vol. 23, No. 8, 085304, 08.2012.

Research output: Contribution to journalArticle

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