Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy

Jennifer L. Miller, Jeremy Woodward, Shaoxia Chen, Mohammed Jaffer, Brandon Weber, Keizo Nagasaki, Yuji Tomaru, Roger Wepf, Alan Roseman, Arvind Varsani, Trevor Sewell

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Heterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and T=3 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a 'bump' on the surface of the protein. Though the capsid protein probably has the classic 'jelly roll' β-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection.

Original languageEnglish (US)
Pages (from-to)1960-1970
Number of pages11
JournalJournal of General Virology
Volume92
Issue number8
DOIs
StatePublished - Aug 2011

ASJC Scopus subject areas

  • Virology

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    Miller, J. L., Woodward, J., Chen, S., Jaffer, M., Weber, B., Nagasaki, K., Tomaru, Y., Wepf, R., Roseman, A., Varsani, A., & Sewell, T. (2011). Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy. Journal of General Virology, 92(8), 1960-1970. https://doi.org/10.1099/vir.0.031211-0