Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae

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Deoxyribonucleic acid (DNA) synthesis was examined in asynchronous and synchronous cultures of a number of cdc (cell division cycle) temperature sensitive mutant strains. The kinetics of DNA synthesis after a shift to the restrictive temperature were compared with that obtained after inhibition of protein synthesis at the permissive temperature, a condition that specifically blocks the initiation of new rounds of DNA replication, but does not block those in progress. Mutations in three genes (cdc 4, 7, and 28) appear to block a precondition for DNA synthesis since cells carrying these lesions cannot start new rounds of DNA replication after a shift from permissive to restrictive temperature, but can finish rounds that were in progress. These three genes are classified as having roles in the 'initiation' of DNA synthesis. Mutations in two genes (cdc 8 and 21) block DNA synthesis itself since cells harboring these lesions that had started DNA synthesis at the permissive temperature arrest synthesis abruptly upon a shift to the restrictive temperature. Mutations in 13 other cdc genes do not impair DNA synthesis in the first cell cycle at the restrictive temperature.

Original languageEnglish (US)
Pages (from-to)966-974
Number of pages9
JournalJournal of bacteriology
Issue number3
StatePublished - Dec 1 1973
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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