Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase

Yuka Ito, Junko Okuda-Shimazaki, Wakako Tsugawa, Noya Loew, Isao Shitanda, Chi En Lin, Jeffrey LaBelle, Koji Sode

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Faradaic electrochemical impedance spectroscopy (faradaic EIS) is an attractive measurement principle for biosensors. However, there have been no reports on sensors employing direct electron transfer (DET)-type redox enzymes based on faradaic EIS principle. In this study, we have attempted to construct the 3rd-generation faradaic enzyme EIS sensor, which used DET-type flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (GDH) complex, to elucidate its characteristic properties as well as to investigate its potential application as the future immunosensor platform. The gold disk electrodes (GDEs) with DET-type FADGDH prepared using self-assembled monolayer (SAM) showed the glucose concentration dependent impedance change, which was confirmed by the change in the charge transfer resistance (R ct ). The Δ(1/R ct ) values were also affected by DC bias potential and the length of SAM. Based on the Nyquist plot and Bode plot simulations, glucose sensing by imaginary impedance monitoring under fixed frequency (5 mHz) was carried out, revealing the higher sensitivity at low glucose concentration with wider linear range (0.02–0.2 mM). Considering this high sensitivity toward glucose, the 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalBiosensors and Bioelectronics
Volume129
DOIs
StatePublished - Mar 15 2019

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Glucose 1-Dehydrogenase
Glucose
Enzyme sensors
Electrons
Dielectric Spectroscopy
Sensors
Self assembled monolayers
Electric Impedance
Electrochemical impedance spectroscopy
Oxidation-Reduction
Enzymes
Immunosensors
Flavin-Adenine Dinucleotide
Biosensing Techniques
Biosensors
Gold
Charge transfer
Electrodes
Oxidoreductases
Monitoring

Keywords

  • Charge transfer resistance
  • Direct electron transfer
  • FAD dependent glucose dehydrogenase complex
  • Faradaic electrochemical impedance spectroscopy
  • Imaginary impedance monitoring
  • Impedimetric biosensor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Ito, Y., Okuda-Shimazaki, J., Tsugawa, W., Loew, N., Shitanda, I., Lin, C. E., ... Sode, K. (2019). Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase. Biosensors and Bioelectronics, 129, 189-197. https://doi.org/10.1016/j.bios.2019.01.018

Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase. / Ito, Yuka; Okuda-Shimazaki, Junko; Tsugawa, Wakako; Loew, Noya; Shitanda, Isao; Lin, Chi En; LaBelle, Jeffrey; Sode, Koji.

In: Biosensors and Bioelectronics, Vol. 129, 15.03.2019, p. 189-197.

Research output: Contribution to journalArticle

Ito, Y, Okuda-Shimazaki, J, Tsugawa, W, Loew, N, Shitanda, I, Lin, CE, LaBelle, J & Sode, K 2019, 'Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase', Biosensors and Bioelectronics, vol. 129, pp. 189-197. https://doi.org/10.1016/j.bios.2019.01.018
Ito, Yuka ; Okuda-Shimazaki, Junko ; Tsugawa, Wakako ; Loew, Noya ; Shitanda, Isao ; Lin, Chi En ; LaBelle, Jeffrey ; Sode, Koji. / Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase. In: Biosensors and Bioelectronics. 2019 ; Vol. 129. pp. 189-197.
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