TY - JOUR
T1 - Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase
AU - Ito, Yuka
AU - Okuda-Shimazaki, Junko
AU - Tsugawa, Wakako
AU - Loew, Noya
AU - Shitanda, Isao
AU - Lin, Chi En
AU - LaBelle, Jeffrey
AU - Sode, Koji
N1 - Funding Information:
Y. I. was supported by the Japan Student Services Organisation ( JASSO ) Student Exchange Support Program (Scholarship for Short-term study abroad, FY2016) to research at Arizona State University.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/3/15
Y1 - 2019/3/15
N2 - Faradaic electrochemical impedance spectroscopy (faradaic EIS) is an attractive measurement principle for biosensors. However, there have been no reports on sensors employing direct electron transfer (DET)-type redox enzymes based on faradaic EIS principle. In this study, we have attempted to construct the 3rd-generation faradaic enzyme EIS sensor, which used DET-type flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (GDH) complex, to elucidate its characteristic properties as well as to investigate its potential application as the future immunosensor platform. The gold disk electrodes (GDEs) with DET-type FADGDH prepared using self-assembled monolayer (SAM) showed the glucose concentration dependent impedance change, which was confirmed by the change in the charge transfer resistance (R ct ). The Δ(1/R ct ) values were also affected by DC bias potential and the length of SAM. Based on the Nyquist plot and Bode plot simulations, glucose sensing by imaginary impedance monitoring under fixed frequency (5 mHz) was carried out, revealing the higher sensitivity at low glucose concentration with wider linear range (0.02–0.2 mM). Considering this high sensitivity toward glucose, the 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe.
AB - Faradaic electrochemical impedance spectroscopy (faradaic EIS) is an attractive measurement principle for biosensors. However, there have been no reports on sensors employing direct electron transfer (DET)-type redox enzymes based on faradaic EIS principle. In this study, we have attempted to construct the 3rd-generation faradaic enzyme EIS sensor, which used DET-type flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (GDH) complex, to elucidate its characteristic properties as well as to investigate its potential application as the future immunosensor platform. The gold disk electrodes (GDEs) with DET-type FADGDH prepared using self-assembled monolayer (SAM) showed the glucose concentration dependent impedance change, which was confirmed by the change in the charge transfer resistance (R ct ). The Δ(1/R ct ) values were also affected by DC bias potential and the length of SAM. Based on the Nyquist plot and Bode plot simulations, glucose sensing by imaginary impedance monitoring under fixed frequency (5 mHz) was carried out, revealing the higher sensitivity at low glucose concentration with wider linear range (0.02–0.2 mM). Considering this high sensitivity toward glucose, the 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe.
KW - Charge transfer resistance
KW - Direct electron transfer
KW - FAD dependent glucose dehydrogenase complex
KW - Faradaic electrochemical impedance spectroscopy
KW - Imaginary impedance monitoring
KW - Impedimetric biosensor
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U2 - 10.1016/j.bios.2019.01.018
DO - 10.1016/j.bios.2019.01.018
M3 - Article
C2 - 30721794
AN - SCOPUS:85060894540
SN - 0956-5663
VL - 129
SP - 189
EP - 197
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -