TY - JOUR
T1 - Thermoreversible copolymer gels for extracellular matrix
AU - Vernon, Brent
AU - Kim, Sung Wan
AU - Bae, You Han
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - To improve the properties of a reversible synthetic extracellular matrix based on a thermally reversible polymer, copolymers of N-isopropylacrylamide and acrylic acid were prepared in benzene with varying contents of acrylic acid (0 to 3%) and the thermal properties were evaluated. The poly(N- isopropylacrylamide) and copolymers made with acrylic acid had molecular weights from 0.8 to 1.7 x 106 D. Differential scanning calorimetry (DSC) showed the high-molecular-weight acrylic acid copolymers had similar onset temperatures to the homopolymers, but the peak width was considerably increased with increasing acrylic acid content. DSC and cloud point measurements showed that polymers with 0 to 3% acrylic acid exhibit a lower critical solution temperature (LCST) transition between 30°and 37°C. In swelling studies, the homopolymer showed significant syneresis at temperatures above 31°C. Copolymers with 1 and 1.5% showed syneresis beginning at 32°and 37°C, respectively. At 37°C the copolymers with 1.5-3% acrylic acid showed little or no syneresis. Due to the high water content and a transition near physiologic conditions (below 37°C), the polymers with 1.5-2.0% acrylic acid exhibited properties that would be useful in the development of a refillable synthetic extracellular matrix. Such a matrix could be applied to several cell types, including islets of Langerhans, for a biohybrid artificial pancreas. (C) 2000 John Wiley and Sons, Inc.
AB - To improve the properties of a reversible synthetic extracellular matrix based on a thermally reversible polymer, copolymers of N-isopropylacrylamide and acrylic acid were prepared in benzene with varying contents of acrylic acid (0 to 3%) and the thermal properties were evaluated. The poly(N- isopropylacrylamide) and copolymers made with acrylic acid had molecular weights from 0.8 to 1.7 x 106 D. Differential scanning calorimetry (DSC) showed the high-molecular-weight acrylic acid copolymers had similar onset temperatures to the homopolymers, but the peak width was considerably increased with increasing acrylic acid content. DSC and cloud point measurements showed that polymers with 0 to 3% acrylic acid exhibit a lower critical solution temperature (LCST) transition between 30°and 37°C. In swelling studies, the homopolymer showed significant syneresis at temperatures above 31°C. Copolymers with 1 and 1.5% showed syneresis beginning at 32°and 37°C, respectively. At 37°C the copolymers with 1.5-3% acrylic acid showed little or no syneresis. Due to the high water content and a transition near physiologic conditions (below 37°C), the polymers with 1.5-2.0% acrylic acid exhibited properties that would be useful in the development of a refillable synthetic extracellular matrix. Such a matrix could be applied to several cell types, including islets of Langerhans, for a biohybrid artificial pancreas. (C) 2000 John Wiley and Sons, Inc.
KW - Acrylic acid
KW - Biohybrid artificial pancreas
KW - N-isopropylacrylamide
KW - Thermal reversibility
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U2 - 10.1002/(SICI)1097-4636(200007)51:1<69::AID-JBM10>3.0.CO;2-6
DO - 10.1002/(SICI)1097-4636(200007)51:1<69::AID-JBM10>3.0.CO;2-6
M3 - Article
C2 - 10813747
AN - SCOPUS:0343294322
SN - 0021-9304
VL - 51
SP - 69
EP - 79
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 1
ER -