Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds

Karime Jocelyn Rosas Gomez, Amrita Pal, Karolena Lein, Brent Vernon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: N-isopropylacrylamide (NIPAAm) copolymers have a lower critical solution temperature (LCST) due to their amphiphilic nature, which allows them to be injectable below LCST and to solidify in situ above the LCST. [1] The aim of this study was to investigate amino acid substitution in an enzyme degradable side group of a NIPAAm copolymer for drug delivery and bioresorbable scaffolds for tissue engineering. Therefore, in this work, a series of NIPAAm-based copolymers with hydrophobic side groups containing the Ala-Pro-Gly-Leu collagenase substrate peptide sequence were synthesized as in situ forming, injectable copolymers. Collagenase is a matrix metalloproteinase (MMP) that plays an important role enabling cell migration and tissue remodelling. [2] The Gly-Leu peptide bond in these polypeptides is cleaved by collagenase, converting the side group into the more hydrophilic GAPG-COOH, thus increasing the LCST of the hydrogel. [1] The side groups Gly-Ala-Pro-Gly-Leu-Phe-NH2 (GAPGLF-NH2), Gly-Ala-Pro-Gly-Leu-Leu-NH2 (GAPGLL-NH2), and Gly-Ala-Pro-Gly-Leu-Val-NH2 (GAPGLV-NH2) were used to synthesize poly(NIPAAm-co-peptide) copolymers.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
Publication statusPublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Gomez, K. J. R., Pal, A., Lein, K., & Vernon, B. (2019). Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.