Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds

Karime Jocelyn Rosas Gomez, Amrita Pal, Karolena Lein, Brent Vernon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: N-isopropylacrylamide (NIPAAm) copolymers have a lower critical solution temperature (LCST) due to their amphiphilic nature, which allows them to be injectable below LCST and to solidify in situ above the LCST. [1] The aim of this study was to investigate amino acid substitution in an enzyme degradable side group of a NIPAAm copolymer for drug delivery and bioresorbable scaffolds for tissue engineering. Therefore, in this work, a series of NIPAAm-based copolymers with hydrophobic side groups containing the Ala-Pro-Gly-Leu collagenase substrate peptide sequence were synthesized as in situ forming, injectable copolymers. Collagenase is a matrix metalloproteinase (MMP) that plays an important role enabling cell migration and tissue remodelling. [2] The Gly-Leu peptide bond in these polypeptides is cleaved by collagenase, converting the side group into the more hydrophilic GAPG-COOH, thus increasing the LCST of the hydrogel. [1] The side groups Gly-Ala-Pro-Gly-Leu-Phe-NH2 (GAPGLF-NH2), Gly-Ala-Pro-Gly-Leu-Leu-NH2 (GAPGLL-NH2), and Gly-Ala-Pro-Gly-Leu-Val-NH2 (GAPGLV-NH2) were used to synthesize poly(NIPAAm-co-peptide) copolymers.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

Fingerprint

Scaffolds (biology)
Drug delivery
Scaffolds
Collagenases
Copolymers
Enzymes
Peptides
Temperature
Pharmaceutical Preparations
glycylleucine
MSH Release-Inhibiting Hormone
Injections
Hydrogel
Amino Acid Substitution
Tissue Engineering
Matrix Metalloproteinases
Polypeptides
Cell Movement
Tissue engineering
Hydrogels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Gomez, K. J. R., Pal, A., Lein, K., & Vernon, B. (2019). Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.

Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds. / Gomez, Karime Jocelyn Rosas; Pal, Amrita; Lein, Karolena; Vernon, Brent.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gomez, KJR, Pal, A, Lein, K & Vernon, B 2019, Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Society for Biomaterials, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 4/3/19.
Gomez KJR, Pal A, Lein K, Vernon B. Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials. 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
Gomez, Karime Jocelyn Rosas ; Pal, Amrita ; Lein, Karolena ; Vernon, Brent. / Thermo-responsive copolymers for enzyme-triggered drug delivery and bioresorbable scaffolds. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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