Thermal inactivation of MS2 bacteriophage as a surrogate of enteric viruses in cow milk

Seyed Reza Sadat Hosseini, Mohammad Reza Edalatian Dovom, Masoud Yavarmanesh, Morteza Abbaszadegan

Research output: Contribution to journalArticle

Abstract

This study examined the effect of 4 thermal processing methods on inactivation of male-specific bacteriophage MS2 in cow milk. MS2 was used as a viral surrogate of enteric viruses in this inactivation study. Milk samples were spiked at 10, 103, 105, and 107 PFU/ml with a milk fat content of 1.5, 2.5 and 3% weight per volume. We found that with higher milk fat content, survival of MS2 increased in the samples spiked with 105 or 107 PFU/ml, indicating that increasing the milk fat content had a significant effect on the survival of MS2 (p < 0.05) in the thermally processed samples. The inactivation results suggest that the most effective thermal processing for viral inactivation in cow milk was having a high temperature long time (HTLT: 85 °C for 30 min) followed by boiling for 2 min. The least effective thermal processing was low temperature for a long time. Therefore, inactivation of enteric viruses in dairy products with a high level of contamination can be effectively achieved under severe thermal processing such as HTLT, or boiling.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalJournal fur Verbraucherschutz und Lebensmittelsicherheit
DOIs
StateAccepted/In press - Jul 27 2017

Fingerprint

Levivirus
Enterovirus
heat inactivation
bacteriophages
inactivation
Milk
Hot Temperature
milk
heat treatment
milk fat
Virus Inactivation
lipid content
boiling
Fats
Temperature
processing technology
Dairy Products
sampling
dairy products
temperature

Keywords

  • Cow milk
  • Enteric viruses
  • MS2 bacteriophage
  • Thermal processing

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Food Animals
  • Agronomy and Crop Science

Cite this

Thermal inactivation of MS2 bacteriophage as a surrogate of enteric viruses in cow milk. / Sadat Hosseini, Seyed Reza; Edalatian Dovom, Mohammad Reza; Yavarmanesh, Masoud; Abbaszadegan, Morteza.

In: Journal fur Verbraucherschutz und Lebensmittelsicherheit, 27.07.2017, p. 1-7.

Research output: Contribution to journalArticle

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