Theoretical analysis of dynamic force spectroscopy experiments on ligand-receptor complexes

M. Raible, M. Evstigneev, P. Reimann, F. W. Bartels, R. Ros

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The forced rupture of single chemical bonds in biomolecular compounds (e.g. ligand-receptor systems) as observed in dynamic force spectroscopy experiments is addressed. Under the assumption that the probability of bond rupture depends only on the instantaneously acting force, a data collapse onto a single master curve is predicted. For rupture data obtained experimentally by dynamic AFM force spectroscopy of a ligand-receptor bond between a DNA and a regulatory protein we do not find such a collapse. We conclude that the above mentioned, generally accepted assumption is not satisfied and we discuss possible explanations.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalJournal of Biotechnology
Volume112
Issue number1-2
DOIs
StatePublished - Aug 26 2004
Externally publishedYes

Keywords

  • Chemical reaction kinetics
  • Single molecule force spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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