The zebrafish xenograft models for investigating cancer and cancer therapeutics

John T. Gamble; Daniel J. Elson; Juliet A. Greenwood; Robyn L. Tanguay; Siva K. Kolluri

doi:10.3390/biology10040252

The zebrafish xenograft models for investigating cancer and cancer therapeutics

John T. Gamble, Daniel J. Elson, Juliet A. Greenwood, Robyn L. Tanguay, Siva K. Kolluri

Mathematical and Natural Sciences, School of (SMNS)

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

In order to develop new cancer therapeutics, rapid, reliable, and relevant biological models are required to screen and validate drug candidates for both efficacy and safety. In recent years, the zebrafish (Danio rerio) has emerged as an excellent model organism suited for these goals. Larval fish or immunocompromised adult fish are used to engraft human cancer cells and serve as a platform for screening potential drug candidates. With zebrafish sharing ~80% of disease-related orthologous genes with humans, they provide a low cost, high-throughput alternative to mouse xenografts that is relevant to human biology. In this review, we provide background on the methods and utility of zebrafish xenograft models in cancer research.

Original language	English (US)
Article number	252
Journal	Biology
Volume	10
Issue number	4
DOIs	https://doi.org/10.3390/biology10040252
State	Published - Apr 2021

Keywords

Cancer therapeutics
Drug discovery
Drug screening
Toxicity testing
Xenograft
Zebrafish

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Agricultural and Biological Sciences

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10.3390/biology10040252

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title = "The zebrafish xenograft models for investigating cancer and cancer therapeutics",

abstract = "In order to develop new cancer therapeutics, rapid, reliable, and relevant biological models are required to screen and validate drug candidates for both efficacy and safety. In recent years, the zebrafish (Danio rerio) has emerged as an excellent model organism suited for these goals. Larval fish or immunocompromised adult fish are used to engraft human cancer cells and serve as a platform for screening potential drug candidates. With zebrafish sharing ~80% of disease-related orthologous genes with humans, they provide a low cost, high-throughput alternative to mouse xenografts that is relevant to human biology. In this review, we provide background on the methods and utility of zebrafish xenograft models in cancer research.",

keywords = "Cancer therapeutics, Drug discovery, Drug screening, Toxicity testing, Xenograft, Zebrafish",

author = "Gamble, {John T.} and Elson, {Daniel J.} and Greenwood, {Juliet A.} and Tanguay, {Robyn L.} and Kolluri, {Siva K.}",

note = "Funding Information: Funding: This research was funded by National Institute of Environmental Health Sciences (NIEHS) [P30 ES030287 and T32 ES007060], National Cancer Institute (CA249627) and by the US Army Medical Research and Material Command (W81XWH-12-1-0069) and American Cancer Society (RSG-13-132-01-CDD). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = apr,

doi = "10.3390/biology10040252",

language = "English (US)",

volume = "10",

journal = "Biology",

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AU - Gamble, John T.

AU - Elson, Daniel J.

AU - Greenwood, Juliet A.

AU - Tanguay, Robyn L.

AU - Kolluri, Siva K.

N1 - Funding Information: Funding: This research was funded by National Institute of Environmental Health Sciences (NIEHS) [P30 ES030287 and T32 ES007060], National Cancer Institute (CA249627) and by the US Army Medical Research and Material Command (W81XWH-12-1-0069) and American Cancer Society (RSG-13-132-01-CDD). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/4

Y1 - 2021/4

N2 - In order to develop new cancer therapeutics, rapid, reliable, and relevant biological models are required to screen and validate drug candidates for both efficacy and safety. In recent years, the zebrafish (Danio rerio) has emerged as an excellent model organism suited for these goals. Larval fish or immunocompromised adult fish are used to engraft human cancer cells and serve as a platform for screening potential drug candidates. With zebrafish sharing ~80% of disease-related orthologous genes with humans, they provide a low cost, high-throughput alternative to mouse xenografts that is relevant to human biology. In this review, we provide background on the methods and utility of zebrafish xenograft models in cancer research.

AB - In order to develop new cancer therapeutics, rapid, reliable, and relevant biological models are required to screen and validate drug candidates for both efficacy and safety. In recent years, the zebrafish (Danio rerio) has emerged as an excellent model organism suited for these goals. Larval fish or immunocompromised adult fish are used to engraft human cancer cells and serve as a platform for screening potential drug candidates. With zebrafish sharing ~80% of disease-related orthologous genes with humans, they provide a low cost, high-throughput alternative to mouse xenografts that is relevant to human biology. In this review, we provide background on the methods and utility of zebrafish xenograft models in cancer research.

KW - Cancer therapeutics

KW - Drug discovery

KW - Drug screening

KW - Toxicity testing

KW - Xenograft

KW - Zebrafish

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The zebrafish xenograft models for investigating cancer and cancer therapeutics

Abstract

Keywords

ASJC Scopus subject areas

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