The zeaxanthin biosynthesis enzyme β-carotene hydroxylase is involved in myxoxanthophyll synthesis in Synechocystis sp. PCC 6803

Delphine Lagarde, Willem Vermaas

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

β-carotene hydroxylase is known to be involved in zeaxanthin synthesis. Disruption of the crtR gene encoding β-carotene hydroxylase in Synechocystis sp. PCC 6803 resulted in the absence of both zeaxanthin synthesis and myxoxanthophyll accumulation in the mutant strain. A new carotenoid was detected in this strain. Its chemical structure was close to that of myxoxanthophyll, but the hydroxyl group on the β-ring was lacking. This compound, deoxy-myxoxanthophyll, most likely is an intermediate in the myxoxanthophyll biosynthesis pathway. Therefore, β-carotene hydroxylase is involved not only in zeaxanthin synthesis but also in myxoxanthophyll synthesis in Synechocystis. Furthermore, myxoxanthophyll in Synechocystis was found to have a higher molecular mass than what was determined in other species. This difference is likely to be due to a difference in the sugar moiety. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalFEBS Letters
Volume454
Issue number3
DOIs
StatePublished - Jul 9 1999

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Synechocystis
Biosynthesis
Carotenoids
Mixed Function Oxygenases
Enzymes
Gene encoding
Molecular mass
Sugars
Hydroxyl Radical
Zeaxanthins
Genes

Keywords

  • Carotenoid
  • Myxoxanthophyll
  • Synechocystis sp. PCC 6803

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The zeaxanthin biosynthesis enzyme β-carotene hydroxylase is involved in myxoxanthophyll synthesis in Synechocystis sp. PCC 6803. / Lagarde, Delphine; Vermaas, Willem.

In: FEBS Letters, Vol. 454, No. 3, 09.07.1999, p. 247-251.

Research output: Contribution to journalArticle

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AB - β-carotene hydroxylase is known to be involved in zeaxanthin synthesis. Disruption of the crtR gene encoding β-carotene hydroxylase in Synechocystis sp. PCC 6803 resulted in the absence of both zeaxanthin synthesis and myxoxanthophyll accumulation in the mutant strain. A new carotenoid was detected in this strain. Its chemical structure was close to that of myxoxanthophyll, but the hydroxyl group on the β-ring was lacking. This compound, deoxy-myxoxanthophyll, most likely is an intermediate in the myxoxanthophyll biosynthesis pathway. Therefore, β-carotene hydroxylase is involved not only in zeaxanthin synthesis but also in myxoxanthophyll synthesis in Synechocystis. Furthermore, myxoxanthophyll in Synechocystis was found to have a higher molecular mass than what was determined in other species. This difference is likely to be due to a difference in the sugar moiety. Copyright (C) 1999 Federation of European Biochemical Societies.

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