The yeast CLC chloride channel functions in cation homeostasis

Roberto A. Gaxiola, Daniel S. Yuan, Richard D. Klausner, Gerald R. Fink

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142-1479 A defect in the yeast GEF1 gene, a CLC chloride channel homolog leads to an iron requirement and cation sensitivity. The iron requirement is due to a failure to load Cu2+ onto a component of the iron uptake system, Fet3. This process, which requires both Gef1 and the Menkes disease Cu2+-ATPase yeast homolog Ccc2, occurs in late- or post-Golgi vesicles, where Gef1 and Ccc2 are localized. The defects of gef1 mutants can be suppressed by the introduction of Torpedo marmorata CLC-0 or Arabidopsis thaliana CLC-c and -d chloride channel genes. The functions of Gef1 in cation homeostasis provide clues to the understanding of diseases caused by chloride channel mutations in humans and cation toxicity in plants.

Original languageEnglish (US)
Pages (from-to)4046-4050
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number7
DOIs
StatePublished - Mar 31 1998

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