@article{fbfd3019244442fe8d91835cc1135ec3,
title = "The XIST Noncoding RNA Functions Independently of BRCA1 in X Inactivation",
abstract = "Females with germline mutations in BRCA1 are predisposed to develop breast and ovarian cancers. A previous report indicated that BRCA1 colocalizes with and is necessary for the correct localization of XIST, a noncoding RNA that coats the inactive X chromosome (Xi) to mediate formation of facultative heterochromatin. A model emerged from this study suggesting that loss of BRCA1 in female cells could reactivate genes on the Xi through loss of the XIST RNA. However, our independent studies of BRCA1 and XIST RNA revealed little evidence to support this model. We report that BRCA1 is not enriched on XIST RNA-coated chromatin of the Xi. Neither mutation nor depletion of BRCA1 causes significant changes in XIST RNA localization or X-linked gene expression. Together, these results do not support a role for BRCA1 in promoting XIST RNA localization to the Xi or regulating XIST-dependent functions in maintaining the stability of facultative heterochromatin.",
author = "Cuiying Xiao and Sharp, {Judith A.} and Misako Kawahara and Davalos, {Albert R.} and Difilippantonio, {Michael J.} and Ying Hu and Wenmei Li and Liu Cao and Ken Buetow and Thomas Ried and Chadwick, {Brian P.} and Deng, {Chu Xia} and Barbara Panning",
note = "Funding Information: J.A.S. and B.P. thank A. Andersen, E. Campeau, L. Chu, H. Cohen, C. de la Cruz, T. Fazzio, J. Huff, D. Nusinow, and M. Royce-Tolland for discussions, reagents, and technical advice. We also thank E. Blackburn, P. O'Farrell, T. Tlsty, and Z. Werb for helpful discussions. HMEC-t cells were obtained from M. Stampfer (LBNL). Vector- and BRCA1-reconstituted HCC1937 cell lines were provided by R. Scully (Harvard Medical School). J.A.S. is a Pfizer Fellow of the Life Science Research Foundation. B.P. is a Pew Scholar. This work was supported by CABCRP grant 11IB-0153 to A.R.D. and NIH grant RO1 GM63671 and a Sandler grant to B.P. Funding Information: C.X. and C.-X.D. thank E. Heard for the mouse Xist probe and the RNA-FISH protocol, C. Brown for the human XIST probe, and F. Xia for BRCA1 - and pcDNA3-reconstituted HCC1937 cell lines. We also thank X. Xu, R.-H. Wang, W. Qiao, and Y. Tominaga of the Deng lab for providing materials for this study. We are grateful to W. Chen at the NIDDK Genomics Core Laboratory for helping with the submission of microarray data. This work was supported by the Intramural Research Program of the NIDDK (NIH). ",
year = "2007",
month = mar,
day = "9",
doi = "10.1016/j.cell.2007.01.034",
language = "English (US)",
volume = "128",
pages = "977--989",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",
}