The use of microparticle injection to introduce genes into animal cells in vitro and in vivo.

Stephen Johnston, D. C. Tang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The biolistic technology has had a tremendous impact on plant and microbial research and development. The experience to date indicates that it should also have important uses with animal applications. The in-chamber system should prove increasingly valuable for transfecting non-dividing cells, primary cells, and other difficult-to-transfect targets. The hand-held version is currently useful for in situ transfection of cells in skin, but has limited effectiveness for internal organ transfection. This limitation may be lessened with further improvements in gun design and microprojectile coating. For gene therapy the most promising applications of this technology appear to be for genetic immunization and protocols which elicit an anti-tumor response.

Original languageEnglish (US)
Pages (from-to)225-236
Number of pages12
JournalGenetic engineering
Volume15
StatePublished - 1993
Externally publishedYes

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Injections
Transfection
Biolistics
Genes
Technology
Firearms
Genetic Therapy
Immunization
Hand
Skin
Research
In Vitro Techniques
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The use of microparticle injection to introduce genes into animal cells in vitro and in vivo. / Johnston, Stephen; Tang, D. C.

In: Genetic engineering, Vol. 15, 1993, p. 225-236.

Research output: Contribution to journalArticle

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