The uniqueness of subunit α of mycobacterial F-ATP synthases

An evolutionary variant for niche adaptation

Priya Ragunathan, Hendrik Sielaff, Lavanya Sundararaman, Goran Biuković, Malathy Sony Subramanian Manimekalai, Dhirendra Singh, Subhashri Kundu, Thorsten Wohland, Wayne Frasch, Thomas Dick, Gerhard Grüber

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The F1F0-ATP (F-ATP) synthase is essential for growth of Mycobacterium tuberculosis, the causative agent of tuberculosis (TB). In addition to their synthase function most F-ATP synthases possess an ATP-hydrolase activity, which is coupled to proton-pumping activity. However, the mycobacterial enzyme lacks this reverse activity, but the reason for this deficiency is unclear. Here, we report that a Mycobacterium-specific, 36-amino acid long C-terminal domain in the nucleotide-binding subunit α (Mtα) of F-ATP synthase suppresses its ATPase activity and determined the mechanism of suppression. First, we employed vesicles to show that in intact membrane-embedded mycobacterial F-ATP synthases deletion of the C-terminal domain enabled ATPase and proton-pumping activity. We then generated a heterologous F-ATP synthase model system, which demonstrated that transfer of the mycobacterial C-terminal domain to a standard F-ATP synthase α subunit suppresses ATPase activity. Single-molecule rotation assays indicated that the introduction of this Mycobacterium-specific domain decreased the angular velocity of the power-stroke after ATP binding. Solution X-ray scattering data and NMR results revealed the solution shape of Mtα and the 3D structure of the subunit α C-terminal peptide 521PDEHVEALDEDKLAKEAVKV540 of M. tubercolosis (Mtα(521–540)), respectively. Together with cross-linking studies, the solution structural data lead to a model, in which Mtα(521–540) comes in close proximity with subunit γ residues 104–109, whose interaction may influence the rotation of the camshaft-like subunit γ. Finally, we propose that the unique segment Mtα(514–549), which is accessible at the C terminus of mycobacterial subunit α, is a promising drug epitope.

Original languageEnglish (US)
Pages (from-to)11262-11279
Number of pages18
JournalJournal of Biological Chemistry
Volume292
Issue number27
DOIs
StatePublished - 2017

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Adenosine Triphosphatases
Mycobacterium
Protons
Adenosine Triphosphate
Camshafts
Angular velocity
Hydrolases
X ray scattering
Mycobacterium tuberculosis
F1F0-ATP synthase
Epitopes
Assays
Tuberculosis
Nucleotides
Stroke
Nuclear magnetic resonance
X-Rays
Membranes
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ragunathan, P., Sielaff, H., Sundararaman, L., Biuković, G., Manimekalai, M. S. S., Singh, D., ... Grüber, G. (2017). The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation. Journal of Biological Chemistry, 292(27), 11262-11279. https://doi.org/10.1074/jbc.M117.784959

The uniqueness of subunit α of mycobacterial F-ATP synthases : An evolutionary variant for niche adaptation. / Ragunathan, Priya; Sielaff, Hendrik; Sundararaman, Lavanya; Biuković, Goran; Manimekalai, Malathy Sony Subramanian; Singh, Dhirendra; Kundu, Subhashri; Wohland, Thorsten; Frasch, Wayne; Dick, Thomas; Grüber, Gerhard.

In: Journal of Biological Chemistry, Vol. 292, No. 27, 2017, p. 11262-11279.

Research output: Contribution to journalArticle

Ragunathan, P, Sielaff, H, Sundararaman, L, Biuković, G, Manimekalai, MSS, Singh, D, Kundu, S, Wohland, T, Frasch, W, Dick, T & Grüber, G 2017, 'The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation', Journal of Biological Chemistry, vol. 292, no. 27, pp. 11262-11279. https://doi.org/10.1074/jbc.M117.784959
Ragunathan, Priya ; Sielaff, Hendrik ; Sundararaman, Lavanya ; Biuković, Goran ; Manimekalai, Malathy Sony Subramanian ; Singh, Dhirendra ; Kundu, Subhashri ; Wohland, Thorsten ; Frasch, Wayne ; Dick, Thomas ; Grüber, Gerhard. / The uniqueness of subunit α of mycobacterial F-ATP synthases : An evolutionary variant for niche adaptation. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 27. pp. 11262-11279.
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