Abstract
We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse-chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS-PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precusor form (Mr 54 500) which could be chased into the mature form (Mr 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N-terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (Mr 2542). The initiator Met was preceded by a Shine-Dalgarno sequence, with the correct spacing.
Original language | English (US) |
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Pages (from-to) | 307-310 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 156 |
Issue number | 2 |
DOIs | |
State | Published - Jun 13 1983 |
Externally published | Yes |
Keywords
- DNA sequencing
- E. scherichia coli outer membrane
- OmpF protein
- Signal sequence
- TolC protein
- colicin El
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology