The TolC protein of Escherichia coli K12 is synthesised in a precursor form

Jim Hackett, Rajeev Misra, Peter Reeves

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse-chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS-PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precusor form (Mr 54 500) which could be chased into the mature form (Mr 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N-terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (Mr 2542). The initiator Met was preceded by a Shine-Dalgarno sequence, with the correct spacing.

Original languageEnglish (US)
Pages (from-to)307-310
Number of pages4
JournalFEBS Letters
Volume156
Issue number2
DOIs
StatePublished - Jun 13 1983
Externally publishedYes

Keywords

  • DNA sequencing
  • E. scherichia coli outer membrane
  • OmpF protein
  • Signal sequence
  • TolC protein
  • colicin El

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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