The three-dimensional structure of the cyanobacterium Synechocystis sp. PCC 6803

Allison M L Van De Meene, Martin F. Hohmann-Marriott, Willem Vermaas, Robert Roberson

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

To advance our knowledge of the model cyanobacterium Synechocystis sp. PCC 6803 we investigated the three-dimensional organization of the cytoplasm using standard transmission electron microscopy and electron tomography. Electron tomography allows a resolution of ∼5 nm in all three dimensions, superior to the resolution of most traditional electron microscopy, which is often limited in part by the thickness of the section (70 nm). The thylakoid membrane pairs formed layered sheets that followed the periphery of the cell and converged at various sites near the cytoplasmic membrane. At some of these sites, the margins of thylakoid membranes associated closely along the external surface of rod-like structures termed thylakoid centers, which sometimes traversed nearly the entire periphery of the cell. The thylakoid membranes surrounded the central cytoplasm that contained inclusions such as ribosomes and carboxysomes. Lipid bodies were dispersed throughout the peripheral cytoplasm and often juxtaposed with cytoplasmic and thylakoid membranes suggesting involvement in thylakoid maintenance or biogenesis. Ribosomes were numerous and mainly located throughout the central cytoplasm with some associated with thylakoid and cytoplasmic membranes. Some ribosomes were attached along internal unit-membrane-like sheets located in the central cytoplasm and appeared to be continuous with existing thylakoid membranes. These results present a detailed analysis of the structure of Synechocystis sp. PCC 6803 using high-resolution bioimaging techniques and will allow future evaluation and comparison with gene-deletion mutants.

Original languageEnglish (US)
Pages (from-to)259-270
Number of pages12
JournalArchives of Microbiology
Volume184
Issue number5
DOIs
StatePublished - Jan 2006

Keywords

  • Cyanobacteria
  • Electron tomography
  • Field-emission cryo-scanning electron microscopy
  • Serial section reconstruction
  • Thylakoid membrane biogenesis
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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