The synthesis of surface coat materials in normal and transformed cells

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Abstract

The rate of synthesis and thickness of the surface coat material in a range of virus-transformed and chemically-transformed cell lines were measured by ellipsometry. Cell lines transformed by polyoma virus, SV 40 virus, Rous sarcoma virus and murine sarcoma virus had a significantly thicker coat than the normal parent cells. An increase in the thickness of the cell coat was not a consistent feature of the transformed cell state since this change was not detected in cell lines transformed by methylcholanthrene. The rate of synthesis of the surface coat was significantly faster in transformed cells than in normal cells. Coat synthesis in normal and transformed cells was inhibited rapidly by treatment with cycloheximide. Inhibition of cellular RNA synthesis by actinomycin D produced rapid inhibition of coat synthesis in normal and chemically-transformed cell, but in certain virus-transformed cell lines coat synthesis continued for up to 2 1 2 h. The significance of these changes in the pattern of coat synthesis in transformed cells in relation to their altered surface properties is discussed.

Original languageEnglish (US)
Pages (from-to)264-270
Number of pages7
JournalExperimental Cell Research
Volume77
Issue number1-2
DOIs
StatePublished - Mar 15 1973
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology

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