The study of cellular "microexudates" by ellipsometry and their relationship to the cell coat

George Poste, L. W. Greenham, L. Mallucci, P. Reeve, D. J. Alexander

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The use of ellipsometry, an optical technique for the detection of very thin films, to measure the thickness of the 'microexudate' deposited by mammalian cells on glass and other solid surfaces is described. Significant differences were found in the thickness and the rate of formation of microexudates in different cell types. Cultivation of cells at low temperatures and in medium supplemented with actinomycin D and cycloheximide inhibited the production of cellular microexudates, indicating that these materials are actively synthesized by the cell and do not result merely from passive leakage of macromolecular material as suggested previously. Evidence is reviewed to show that cellular microexudates have a number of properties in common with the cell coat material.

Original languageEnglish (US)
Pages (from-to)303-313
Number of pages11
JournalExperimental Cell Research
Volume78
Issue number2
DOIs
StatePublished - 1973
Externally publishedYes

Fingerprint

Glycocalyx
Dactinomycin
Cycloheximide
Glass
Temperature

ASJC Scopus subject areas

  • Cell Biology

Cite this

The study of cellular "microexudates" by ellipsometry and their relationship to the cell coat. / Poste, George; Greenham, L. W.; Mallucci, L.; Reeve, P.; Alexander, D. J.

In: Experimental Cell Research, Vol. 78, No. 2, 1973, p. 303-313.

Research output: Contribution to journalArticle

Poste, George ; Greenham, L. W. ; Mallucci, L. ; Reeve, P. ; Alexander, D. J. / The study of cellular "microexudates" by ellipsometry and their relationship to the cell coat. In: Experimental Cell Research. 1973 ; Vol. 78, No. 2. pp. 303-313.
@article{6e3c9db26ef94206a0cb26fa013eacf5,
title = "The study of cellular {"}microexudates{"} by ellipsometry and their relationship to the cell coat",
abstract = "The use of ellipsometry, an optical technique for the detection of very thin films, to measure the thickness of the 'microexudate' deposited by mammalian cells on glass and other solid surfaces is described. Significant differences were found in the thickness and the rate of formation of microexudates in different cell types. Cultivation of cells at low temperatures and in medium supplemented with actinomycin D and cycloheximide inhibited the production of cellular microexudates, indicating that these materials are actively synthesized by the cell and do not result merely from passive leakage of macromolecular material as suggested previously. Evidence is reviewed to show that cellular microexudates have a number of properties in common with the cell coat material.",
author = "George Poste and Greenham, {L. W.} and L. Mallucci and P. Reeve and Alexander, {D. J.}",
year = "1973",
doi = "10.1016/0014-4827(73)90073-6",
language = "English (US)",
volume = "78",
pages = "303--313",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The study of cellular "microexudates" by ellipsometry and their relationship to the cell coat

AU - Poste, George

AU - Greenham, L. W.

AU - Mallucci, L.

AU - Reeve, P.

AU - Alexander, D. J.

PY - 1973

Y1 - 1973

N2 - The use of ellipsometry, an optical technique for the detection of very thin films, to measure the thickness of the 'microexudate' deposited by mammalian cells on glass and other solid surfaces is described. Significant differences were found in the thickness and the rate of formation of microexudates in different cell types. Cultivation of cells at low temperatures and in medium supplemented with actinomycin D and cycloheximide inhibited the production of cellular microexudates, indicating that these materials are actively synthesized by the cell and do not result merely from passive leakage of macromolecular material as suggested previously. Evidence is reviewed to show that cellular microexudates have a number of properties in common with the cell coat material.

AB - The use of ellipsometry, an optical technique for the detection of very thin films, to measure the thickness of the 'microexudate' deposited by mammalian cells on glass and other solid surfaces is described. Significant differences were found in the thickness and the rate of formation of microexudates in different cell types. Cultivation of cells at low temperatures and in medium supplemented with actinomycin D and cycloheximide inhibited the production of cellular microexudates, indicating that these materials are actively synthesized by the cell and do not result merely from passive leakage of macromolecular material as suggested previously. Evidence is reviewed to show that cellular microexudates have a number of properties in common with the cell coat material.

UR - http://www.scopus.com/inward/record.url?scp=0015887861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015887861&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(73)90073-6

DO - 10.1016/0014-4827(73)90073-6

M3 - Article

VL - 78

SP - 303

EP - 313

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -