The study of cellular "microexudates" by ellipsometry and their relationship to the cell coat

G. Poste, L. W. Greenham, L. Mallucci, P. Reeve, D. J. Alexander

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The use of ellipsometry, an optical technique for the detection of very thin films, to measure the thickness of the 'microexudate' deposited by mammalian cells on glass and other solid surfaces is described. Significant differences were found in the thickness and the rate of formation of microexudates in different cell types. Cultivation of cells at low temperatures and in medium supplemented with actinomycin D and cycloheximide inhibited the production of cellular microexudates, indicating that these materials are actively synthesized by the cell and do not result merely from passive leakage of macromolecular material as suggested previously. Evidence is reviewed to show that cellular microexudates have a number of properties in common with the cell coat material.

Original languageEnglish (US)
Pages (from-to)303-313
Number of pages11
JournalExperimental Cell Research
Volume78
Issue number2
DOIs
StatePublished - Apr 1973
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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