The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin

Michael J. Spence, Michael T. Henzl, Peter J. Lammers

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A λgt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5′ untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalPlant Molecular Biology
Volume17
Issue number3
DOIs
StatePublished - Sep 1 1991
Externally publishedYes

Keywords

  • 5′ untranslated sequences
  • French bean
  • phytoferritin
  • plants
  • λgt11

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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