The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin

Michael J. Spence, Michael T. Henzl, Peter Lammers

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A λgt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5′ untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalPlant Molecular Biology
Volume17
Issue number3
DOIs
StatePublished - Sep 1991
Externally publishedYes

Fingerprint

Phaseolus
ferritin
storage proteins
Ferritins
Phaseolus vulgaris
Iron
Complementary DNA
iron
Proteins
leaf meristems
Bacteriophages
Meristem
5' Untranslated Regions
pyrimidines
shoot meristems
5' untranslated regions
mice
green beans
messenger RNA
blood serum

Keywords

  • λgt11
  • 5′ untranslated sequences
  • French bean
  • phytoferritin
  • plants

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin. / Spence, Michael J.; Henzl, Michael T.; Lammers, Peter.

In: Plant Molecular Biology, Vol. 17, No. 3, 09.1991, p. 499-504.

Research output: Contribution to journalArticle

Spence, Michael J. ; Henzl, Michael T. ; Lammers, Peter. / The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin. In: Plant Molecular Biology. 1991 ; Vol. 17, No. 3. pp. 499-504.
@article{3c902cc8ba5147189490b92ccbf26ce6,
title = "The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin",
abstract = "A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A λgt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5′ untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.",
keywords = "λgt11, 5′ untranslated sequences, French bean, phytoferritin, plants",
author = "Spence, {Michael J.} and Henzl, {Michael T.} and Peter Lammers",
year = "1991",
month = "9",
doi = "10.1007/BF00040644",
language = "English (US)",
volume = "17",
pages = "499--504",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin

AU - Spence, Michael J.

AU - Henzl, Michael T.

AU - Lammers, Peter

PY - 1991/9

Y1 - 1991/9

N2 - A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A λgt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5′ untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.

AB - A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A λgt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5′ untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure.

KW - λgt11

KW - 5′ untranslated sequences

KW - French bean

KW - phytoferritin

KW - plants

UR - http://www.scopus.com/inward/record.url?scp=0026227806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026227806&partnerID=8YFLogxK

U2 - 10.1007/BF00040644

DO - 10.1007/BF00040644

M3 - Article

C2 - 1884000

AN - SCOPUS:0026227806

VL - 17

SP - 499

EP - 504

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 3

ER -