The structural organization of the PsaC protein in photosystem i from single crystal EPR and x-ray crystallographic studies

Andreas Kamlowski, Arthur Van Der Est, Petra Fromme, Norbert Krauß, Wolf Dieter Schubert, Olaf Klukas, Dietmar Stehlik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In Photosystem I (PS I) the terminal electron accepters, F(A) and F(B), are iron-sulfur (4Fe-4S) centers, which are bound to the stromal subunit PsaC. The orientation of PsaC is determined relative to the whole PS I complex (see Schubert, W.-D. et al. (1995) in From Light to Biosphere (Mathis, P. ed.), Vol. II, pp. 3-10, Kluwer) from which a molecular model for the structure of PsaC within PS I is derived. Two strategies are followed: (i) PS I single crystal EPR data on the orientation of the g tensors of both F(A)- and F(B)- relative to each other and relative to the crystal axes (see preceding paper) are used in conjuction with the central structural part of the bacterial 2 [Fe4S4] ferredoxins, the cysteine binding motifs of which are known to be homologous to those of PsaC; (ii) the same core structure is fitted into the intermediate resolution electron density map of PS I. The PsaC orientation obtained both ways agree well. The local twofold symmetry axis inherent to the ferredoxin model leaves a twofold ambiguity in the structural conclusion. Deviations from this C2-symmetry in the amino acid sequence of PsaC are analyzed with respect to observable properties which would resolve the remaining structural ambiguity. Arguments both for and against F(A) being the distal iron-sulfur center (to F(x)) are discussed.

Original languageEnglish (US)
Pages (from-to)199-213
Number of pages15
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1319
Issue number2-3
DOIs
StatePublished - Apr 11 1997
Externally publishedYes

Fingerprint

Photosystem I Protein Complex
Paramagnetic resonance
X-Rays
Single crystals
Ferredoxins
X rays
Sulfur
Iron
Electrons
Molecular Models
Molecular Structure
Crystal orientation
Tensors
Carrier concentration
Cysteine
Amino Acid Sequence
Light
Amino Acids
Crystals
psaB subunit photosystem I

Keywords

  • EPR
  • F(A)
  • F(B)
  • Photosystem I
  • PsaC protein
  • X-ray crystallography

ASJC Scopus subject areas

  • Biophysics

Cite this

The structural organization of the PsaC protein in photosystem i from single crystal EPR and x-ray crystallographic studies. / Kamlowski, Andreas; Van Der Est, Arthur; Fromme, Petra; Krauß, Norbert; Schubert, Wolf Dieter; Klukas, Olaf; Stehlik, Dietmar.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1319, No. 2-3, 11.04.1997, p. 199-213.

Research output: Contribution to journalArticle

Kamlowski, Andreas ; Van Der Est, Arthur ; Fromme, Petra ; Krauß, Norbert ; Schubert, Wolf Dieter ; Klukas, Olaf ; Stehlik, Dietmar. / The structural organization of the PsaC protein in photosystem i from single crystal EPR and x-ray crystallographic studies. In: Biochimica et Biophysica Acta - Bioenergetics. 1997 ; Vol. 1319, No. 2-3. pp. 199-213.
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AU - Klukas, Olaf

AU - Stehlik, Dietmar

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