The spontaneous mutation rate in the fission yeast Schizosaccharomyces pombe

Ashley Farlow, Hongan Long, Stéphanie Arnoux, Way Sung, Thomas G. Doak, Magnus Nordborg, Michael Lynch

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The rate at which new mutations arise in the genome is a key factor in the evolution and adaptation of species. Here we describe the rate and spectrum of spontaneous mutations for the fission yeast Schizosaccharomyces pombe, a key model organism with many similarities to higher eukaryotes. We undertook an  1700-generation mutation accumulation (MA) experiment with a haploid S. pombe, generating 422 single-base substitutions and 119 insertion-deletion mutations (indels) across the 96 replicates. This equates to a base-substitution mutation rate of 2.00 3 10210 mutations per site per generation, similar to that reported for the distantly related budding yeast Saccharomyces cerevisiae. However, these two yeast species differ dramatically in their spectrum of base substitutions, the types of indels (S. pombe is more prone to insertions), and the pattern of selection required to counteract a strong AT-biased mutation rate. Overall, our results indicate that GC-biased gene conversion does not play a major role in shaping the nucleotide composition of the S. pombe genome and suggest that the mechanisms of DNA maintenance may have diverged significantly between fission and budding yeasts. Unexpectedly, CpG sites appear to be excessively liable to mutation in both species despite the likely absence of DNA methylation.

Original languageEnglish (US)
Pages (from-to)737-744
Number of pages8
JournalGenetics
Volume201
Issue number2
DOIs
StatePublished - Oct 2015
Externally publishedYes

Keywords

  • Biased gene conversion
  • Effective population size
  • Fission yeast
  • Mutation accumulation

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Farlow, A., Long, H., Arnoux, S., Sung, W., Doak, T. G., Nordborg, M., & Lynch, M. (2015). The spontaneous mutation rate in the fission yeast Schizosaccharomyces pombe. Genetics, 201(2), 737-744. https://doi.org/10.1534/genetics.115.177329