The specific growth rate of Pseudomonas putida PAW1 influences the conjugal transfer rate of the TOL plasmid

B. F. Smets, B. E. Rittmann, D. A. Stahl

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Abstract

The kinetics of the conjugal transfer of a TOL plasmid were investigated by using Pseudomonas putida PAW1 as the donor strain and P. aeruginosa PAO 1162 as the recipient strain. Short-term batch mating experiments were performed in a nonselective medium, while the evolution of the different cell types was determined by selective plating techniques. The experimental data were analyzed by using a mass action model that describes plasmid transfer kinetics. This method allowed analysis of the mating experiments by a single intrinsic kinetic parameter for conjugal plasmid transfer. Further results indicated that the specific growth rate of the donor strain antecedent to the mating experiment had a strong impact on the measured intrinsic plasmid transfer rate coefficient, which ranged from 1 x 10-14 to 5 x 10-13 ml per cell per min. Preliminary analysis suggested that the transfer rates of the TOL plasmid are large enough to maintain the TOL plasmid in a dense microbial community without selective pressures.

Original languageEnglish (US)
Pages (from-to)3430-3437
Number of pages8
JournalApplied and environmental microbiology
Volume59
Issue number10
StatePublished - Jan 1 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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