The solubility of hen lysozyme in ethylammonium nitrate/H2O mixtures and a novel approach to protein crystallization

Nolene Byrne, Charles Angell

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at intermediate EAN content. We exploit this solubility profile in a novel approach to generating crystals of hydrophilic proteins, based on rehydration of a high concentration protein solution. We describe the production of crystals of X-ray diffraction quality. Two related ionic liquid solvent systems, with the same solubility profiles but different effective pH characteristics, are identified for future evaluation.

Original languageEnglish (US)
Pages (from-to)793-803
Number of pages11
Issue number2
StatePublished - Feb 2010


  • Protein crystallization
  • Protic ionic liquids
  • Solubility and protein stability

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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