The single particles, clusters and biomolecules and serial femtosecond crystallography instrument of the european XFEL: Initial installation

Adrian P. Mancuso, Andrew Aquila, Lewis Batchelor, Richard J. Bean, Johan Bielecki, Gannon Borchers, Katerina Doerner, Klaus Giewekemeyer, Rita Graceffa, Oliver D. Kelsey, Yoonhee Kim, Henry J. Kirkwood, Alexis Legrand, Romain Letrun, Bradley Manning, Luis Lopez Morillo, Marc Messerschmidt, Grant Mills, Steffen Raabe, Nadja ReimersAdam Round, Tokushi Sato, Joachim Schulz, Cedric Signe Takem, Marcin Sikorski, Stephan Stern, Prasad Thute, Patrik Vagovič, Britta Weinhausen, Thomas Tschentscher

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The European X-ray Free-Electron Laser (FEL) became the first operational high-repetition-rate hard X-ray FEL with first lasing in May 2017. Biological structure determination has already benefitted from the unique properties and capabilities of X-ray FELs, predominantly through the development and application of serial crystallography. The possibility of now performing such experiments at data rates more than an order of magnitude greater than previous X-ray FELs enables not only a higher rate of discovery but also new classes of experiments previously not feasible at lower data rates. One example is time-resolved experiments requiring a higher number of time steps for interpretation, or structure determination from samples with low hit rates in conventional X-ray FEL serial crystallography. Following first lasing at the European XFEL, initial commissioning and operation occurred at two scientific instruments, one of which is the Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) instrument. This instrument provides a photon energy range, focal spot sizes and diagnostic tools necessary for structure determination of biological specimens. The instrumentation explicitly addresses serial crystallography and the developing single particle imaging method as well as other forward-scattering and diffraction techniques. This paper describes the major science cases of SPB/SFX and its initial instrumentation – in particular its optical systems, available sample delivery methods, 2D detectors, supporting optical laser systems and key diagnostic components. The present capabilities of the instrument will be reviewed and a brief outlook of its future capabilities is also described.

Original languageEnglish (US)
Pages (from-to)660-676
Number of pages17
JournalJournal of synchrotron radiation
Volume26
Issue number3
DOIs
StatePublished - May 1 2019

Fingerprint

Crystallography
Free electron lasers
Biomolecules
crystallography
installing
X ray lasers
free electron lasers
x rays
lasing
Forward scattering
X rays
Experiments
Optical systems
forward scattering
Photons
Diffraction
repetition
delivery
Detectors
Imaging techniques

Keywords

  • Instrumentation
  • Serial crystallography
  • XFEL

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

The single particles, clusters and biomolecules and serial femtosecond crystallography instrument of the european XFEL : Initial installation. / Mancuso, Adrian P.; Aquila, Andrew; Batchelor, Lewis; Bean, Richard J.; Bielecki, Johan; Borchers, Gannon; Doerner, Katerina; Giewekemeyer, Klaus; Graceffa, Rita; Kelsey, Oliver D.; Kim, Yoonhee; Kirkwood, Henry J.; Legrand, Alexis; Letrun, Romain; Manning, Bradley; Morillo, Luis Lopez; Messerschmidt, Marc; Mills, Grant; Raabe, Steffen; Reimers, Nadja; Round, Adam; Sato, Tokushi; Schulz, Joachim; Takem, Cedric Signe; Sikorski, Marcin; Stern, Stephan; Thute, Prasad; Vagovič, Patrik; Weinhausen, Britta; Tschentscher, Thomas.

In: Journal of synchrotron radiation, Vol. 26, No. 3, 01.05.2019, p. 660-676.

Research output: Contribution to journalArticle

Mancuso, AP, Aquila, A, Batchelor, L, Bean, RJ, Bielecki, J, Borchers, G, Doerner, K, Giewekemeyer, K, Graceffa, R, Kelsey, OD, Kim, Y, Kirkwood, HJ, Legrand, A, Letrun, R, Manning, B, Morillo, LL, Messerschmidt, M, Mills, G, Raabe, S, Reimers, N, Round, A, Sato, T, Schulz, J, Takem, CS, Sikorski, M, Stern, S, Thute, P, Vagovič, P, Weinhausen, B & Tschentscher, T 2019, 'The single particles, clusters and biomolecules and serial femtosecond crystallography instrument of the european XFEL: Initial installation', Journal of synchrotron radiation, vol. 26, no. 3, pp. 660-676. https://doi.org/10.1107/S1600577519003308
Mancuso, Adrian P. ; Aquila, Andrew ; Batchelor, Lewis ; Bean, Richard J. ; Bielecki, Johan ; Borchers, Gannon ; Doerner, Katerina ; Giewekemeyer, Klaus ; Graceffa, Rita ; Kelsey, Oliver D. ; Kim, Yoonhee ; Kirkwood, Henry J. ; Legrand, Alexis ; Letrun, Romain ; Manning, Bradley ; Morillo, Luis Lopez ; Messerschmidt, Marc ; Mills, Grant ; Raabe, Steffen ; Reimers, Nadja ; Round, Adam ; Sato, Tokushi ; Schulz, Joachim ; Takem, Cedric Signe ; Sikorski, Marcin ; Stern, Stephan ; Thute, Prasad ; Vagovič, Patrik ; Weinhausen, Britta ; Tschentscher, Thomas. / The single particles, clusters and biomolecules and serial femtosecond crystallography instrument of the european XFEL : Initial installation. In: Journal of synchrotron radiation. 2019 ; Vol. 26, No. 3. pp. 660-676.
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AU - Bean, Richard J.

AU - Bielecki, Johan

AU - Borchers, Gannon

AU - Doerner, Katerina

AU - Giewekemeyer, Klaus

AU - Graceffa, Rita

AU - Kelsey, Oliver D.

AU - Kim, Yoonhee

AU - Kirkwood, Henry J.

AU - Legrand, Alexis

AU - Letrun, Romain

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AU - Morillo, Luis Lopez

AU - Messerschmidt, Marc

AU - Mills, Grant

AU - Raabe, Steffen

AU - Reimers, Nadja

AU - Round, Adam

AU - Sato, Tokushi

AU - Schulz, Joachim

AU - Takem, Cedric Signe

AU - Sikorski, Marcin

AU - Stern, Stephan

AU - Thute, Prasad

AU - Vagovič, Patrik

AU - Weinhausen, Britta

AU - Tschentscher, Thomas

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