The Salmonella pathogenicity island (SPI) I contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium

Yakhya Dieye, Keith Ameiss, Melha Mellata, Roy Curtiss

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background. Salmonella enterica serovar Typhimurium (Typhimurium) is an important pathogen that infects a broad range of hosts. In humans, Typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. Typhimurium infection occurs mainly through the ingestion of contaminated food including poultry, pork, eggs, and milk. Chickens that are asymptomatic carriers of Typhimurium constitute a potential reservoir for infection. The type three secretion systems encoded by Salmonella pathogenicity islands (SPI) 1 and 2 are major virulence factors of Salmonella. However, only a few studies have investigated their role during the infection of chickens. Results. We have taken a mixed infection approach to study the contribution of SPI1 and SPI2 to the colonization of the chicken by Typhimurium. We found that SPI1 contributes to colonization of both the cecum and spleen in the chicken. In contrast, SPI2 contributes to colonization of the spleen but not the cecum and, in the absence of SPI1, inhibits cecal colonization. Additionally, we show that the contribution of SPI1 in the spleen is greater than that of SPI2. These results are different from those observed during the infection of the mouse by Typhimurium where SPI2 is the major player during systemic colonization. Conclusion. The co-infection model we used provides a sensitive assay that confirms the role of SPI1 and clarifies the role of SPI2 in the colonization of the chicken by Typhimurium.

Original languageEnglish (US)
Article number3
JournalBMC Microbiology
Volume9
DOIs
StatePublished - 2009

Fingerprint

Genomic Islands
Salmonella enterica
Salmonella
Chickens
Spleen
Cecum
Infection
Coinfection
Host Specificity
Gastroenteritis
Virulence Factors
Poultry
Abdominal Pain
Eggs
Vomiting
Diarrhea
Milk
Eating
Serogroup
Food

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

The Salmonella pathogenicity island (SPI) I contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium. / Dieye, Yakhya; Ameiss, Keith; Mellata, Melha; Curtiss, Roy.

In: BMC Microbiology, Vol. 9, 3, 2009.

Research output: Contribution to journalArticle

@article{5a67cb5e88554815bdf93d1b07983ba3,
title = "The Salmonella pathogenicity island (SPI) I contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium",
abstract = "Background. Salmonella enterica serovar Typhimurium (Typhimurium) is an important pathogen that infects a broad range of hosts. In humans, Typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. Typhimurium infection occurs mainly through the ingestion of contaminated food including poultry, pork, eggs, and milk. Chickens that are asymptomatic carriers of Typhimurium constitute a potential reservoir for infection. The type three secretion systems encoded by Salmonella pathogenicity islands (SPI) 1 and 2 are major virulence factors of Salmonella. However, only a few studies have investigated their role during the infection of chickens. Results. We have taken a mixed infection approach to study the contribution of SPI1 and SPI2 to the colonization of the chicken by Typhimurium. We found that SPI1 contributes to colonization of both the cecum and spleen in the chicken. In contrast, SPI2 contributes to colonization of the spleen but not the cecum and, in the absence of SPI1, inhibits cecal colonization. Additionally, we show that the contribution of SPI1 in the spleen is greater than that of SPI2. These results are different from those observed during the infection of the mouse by Typhimurium where SPI2 is the major player during systemic colonization. Conclusion. The co-infection model we used provides a sensitive assay that confirms the role of SPI1 and clarifies the role of SPI2 in the colonization of the chicken by Typhimurium.",
author = "Yakhya Dieye and Keith Ameiss and Melha Mellata and Roy Curtiss",
year = "2009",
doi = "10.1186/1471-2180-9-3",
language = "English (US)",
volume = "9",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The Salmonella pathogenicity island (SPI) I contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium

AU - Dieye, Yakhya

AU - Ameiss, Keith

AU - Mellata, Melha

AU - Curtiss, Roy

PY - 2009

Y1 - 2009

N2 - Background. Salmonella enterica serovar Typhimurium (Typhimurium) is an important pathogen that infects a broad range of hosts. In humans, Typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. Typhimurium infection occurs mainly through the ingestion of contaminated food including poultry, pork, eggs, and milk. Chickens that are asymptomatic carriers of Typhimurium constitute a potential reservoir for infection. The type three secretion systems encoded by Salmonella pathogenicity islands (SPI) 1 and 2 are major virulence factors of Salmonella. However, only a few studies have investigated their role during the infection of chickens. Results. We have taken a mixed infection approach to study the contribution of SPI1 and SPI2 to the colonization of the chicken by Typhimurium. We found that SPI1 contributes to colonization of both the cecum and spleen in the chicken. In contrast, SPI2 contributes to colonization of the spleen but not the cecum and, in the absence of SPI1, inhibits cecal colonization. Additionally, we show that the contribution of SPI1 in the spleen is greater than that of SPI2. These results are different from those observed during the infection of the mouse by Typhimurium where SPI2 is the major player during systemic colonization. Conclusion. The co-infection model we used provides a sensitive assay that confirms the role of SPI1 and clarifies the role of SPI2 in the colonization of the chicken by Typhimurium.

AB - Background. Salmonella enterica serovar Typhimurium (Typhimurium) is an important pathogen that infects a broad range of hosts. In humans, Typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. Typhimurium infection occurs mainly through the ingestion of contaminated food including poultry, pork, eggs, and milk. Chickens that are asymptomatic carriers of Typhimurium constitute a potential reservoir for infection. The type three secretion systems encoded by Salmonella pathogenicity islands (SPI) 1 and 2 are major virulence factors of Salmonella. However, only a few studies have investigated their role during the infection of chickens. Results. We have taken a mixed infection approach to study the contribution of SPI1 and SPI2 to the colonization of the chicken by Typhimurium. We found that SPI1 contributes to colonization of both the cecum and spleen in the chicken. In contrast, SPI2 contributes to colonization of the spleen but not the cecum and, in the absence of SPI1, inhibits cecal colonization. Additionally, we show that the contribution of SPI1 in the spleen is greater than that of SPI2. These results are different from those observed during the infection of the mouse by Typhimurium where SPI2 is the major player during systemic colonization. Conclusion. The co-infection model we used provides a sensitive assay that confirms the role of SPI1 and clarifies the role of SPI2 in the colonization of the chicken by Typhimurium.

UR - http://www.scopus.com/inward/record.url?scp=60849104824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60849104824&partnerID=8YFLogxK

U2 - 10.1186/1471-2180-9-3

DO - 10.1186/1471-2180-9-3

M3 - Article

C2 - 19126220

AN - SCOPUS:60849104824

VL - 9

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

M1 - 3

ER -