The role of hybridization in the origin and spread of asexuality in Daphnia

Sen Xu, David J. Innes, Michael Lynch, Melania E. Cristescu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The molecular mechanisms leading to asexuality remain little understood despite their substantial bearing on why sexual reproduction is dominant in nature. Here, we examine the role of hybridization in the origin and spread of obligate asexuality in Daphnia pulex, arguably the best-documented case of contagious asexuality. Obligately parthenogenetic (OP) clones of D. pulex have traditionally been separated into 'hybrid' (Ldh SF) and 'nonhybrid' (Ldh SS) forms because the lactase dehydrogenase (Ldh) locus distinguishes the cyclically parthenogenetic (CP) lake dwelling Daphnia pulicaria (Ldh FF) from its ephemeral pond dwelling sister species D. pulex (Ldh SS). The results of our population genetic analyses based on microsatellite loci suggest that both Ldh SS and SF OP individuals can originate from the crossing of CP female F 1 (D. pulex × D. pulicaria) and backcross with males from OP lineages carrying genes that suppress meiosis specifically in female offspring. In previous studies, a suite of diagnostic markers was found to be associated with OP in Ldh SS D. pulex lineages. Our association mapping supports a similar genetic mechanism for the spread of obligate parthenogenesis in Ldh SF OP individuals. Interestingly, our study shows that CP D. pulicaria carry many of the diagnostic microsatellite alleles associated with obligate parthenogenesis. We argue that the assemblage of mutations that suppress meiosis and underlie obligate parthenogenesis in D. pulex originated due to a unique historical hybridization and introgression event between D. pulex and D. pulicaria.

Original languageEnglish (US)
Pages (from-to)4549-4561
Number of pages13
JournalMolecular Ecology
Volume22
Issue number17
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Fingerprint

Lactase
Daphnia
parthenogenesis
Daphnia pulex
beta-galactosidase
Pulicaria
Oxidoreductases
hybridization
Parthenogenesis
ephemeral pool
sexual reproduction
introgression
population genetics
clone
mutation
allele
Meiosis
meiosis
Microsatellite Repeats
gene

Keywords

  • asexuality
  • Daphnia pulex
  • Daphnia pulicaria
  • hybridization
  • meiosis suppression
  • parthenogenesis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

The role of hybridization in the origin and spread of asexuality in Daphnia. / Xu, Sen; Innes, David J.; Lynch, Michael; Cristescu, Melania E.

In: Molecular Ecology, Vol. 22, No. 17, 01.09.2013, p. 4549-4561.

Research output: Contribution to journalArticle

Xu, Sen ; Innes, David J. ; Lynch, Michael ; Cristescu, Melania E. / The role of hybridization in the origin and spread of asexuality in Daphnia. In: Molecular Ecology. 2013 ; Vol. 22, No. 17. pp. 4549-4561.
@article{1b6887ee48b643428924a5a1ca8cdbac,
title = "The role of hybridization in the origin and spread of asexuality in Daphnia",
abstract = "The molecular mechanisms leading to asexuality remain little understood despite their substantial bearing on why sexual reproduction is dominant in nature. Here, we examine the role of hybridization in the origin and spread of obligate asexuality in Daphnia pulex, arguably the best-documented case of contagious asexuality. Obligately parthenogenetic (OP) clones of D. pulex have traditionally been separated into 'hybrid' (Ldh SF) and 'nonhybrid' (Ldh SS) forms because the lactase dehydrogenase (Ldh) locus distinguishes the cyclically parthenogenetic (CP) lake dwelling Daphnia pulicaria (Ldh FF) from its ephemeral pond dwelling sister species D. pulex (Ldh SS). The results of our population genetic analyses based on microsatellite loci suggest that both Ldh SS and SF OP individuals can originate from the crossing of CP female F 1 (D. pulex × D. pulicaria) and backcross with males from OP lineages carrying genes that suppress meiosis specifically in female offspring. In previous studies, a suite of diagnostic markers was found to be associated with OP in Ldh SS D. pulex lineages. Our association mapping supports a similar genetic mechanism for the spread of obligate parthenogenesis in Ldh SF OP individuals. Interestingly, our study shows that CP D. pulicaria carry many of the diagnostic microsatellite alleles associated with obligate parthenogenesis. We argue that the assemblage of mutations that suppress meiosis and underlie obligate parthenogenesis in D. pulex originated due to a unique historical hybridization and introgression event between D. pulex and D. pulicaria.",
keywords = "asexuality, Daphnia pulex, Daphnia pulicaria, hybridization, meiosis suppression, parthenogenesis",
author = "Sen Xu and Innes, {David J.} and Michael Lynch and Cristescu, {Melania E.}",
year = "2013",
month = "9",
day = "1",
doi = "10.1111/mec.12407",
language = "English (US)",
volume = "22",
pages = "4549--4561",
journal = "Molecular Ecology",
issn = "0962-1083",
publisher = "Wiley-Blackwell",
number = "17",

}

TY - JOUR

T1 - The role of hybridization in the origin and spread of asexuality in Daphnia

AU - Xu, Sen

AU - Innes, David J.

AU - Lynch, Michael

AU - Cristescu, Melania E.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - The molecular mechanisms leading to asexuality remain little understood despite their substantial bearing on why sexual reproduction is dominant in nature. Here, we examine the role of hybridization in the origin and spread of obligate asexuality in Daphnia pulex, arguably the best-documented case of contagious asexuality. Obligately parthenogenetic (OP) clones of D. pulex have traditionally been separated into 'hybrid' (Ldh SF) and 'nonhybrid' (Ldh SS) forms because the lactase dehydrogenase (Ldh) locus distinguishes the cyclically parthenogenetic (CP) lake dwelling Daphnia pulicaria (Ldh FF) from its ephemeral pond dwelling sister species D. pulex (Ldh SS). The results of our population genetic analyses based on microsatellite loci suggest that both Ldh SS and SF OP individuals can originate from the crossing of CP female F 1 (D. pulex × D. pulicaria) and backcross with males from OP lineages carrying genes that suppress meiosis specifically in female offspring. In previous studies, a suite of diagnostic markers was found to be associated with OP in Ldh SS D. pulex lineages. Our association mapping supports a similar genetic mechanism for the spread of obligate parthenogenesis in Ldh SF OP individuals. Interestingly, our study shows that CP D. pulicaria carry many of the diagnostic microsatellite alleles associated with obligate parthenogenesis. We argue that the assemblage of mutations that suppress meiosis and underlie obligate parthenogenesis in D. pulex originated due to a unique historical hybridization and introgression event between D. pulex and D. pulicaria.

AB - The molecular mechanisms leading to asexuality remain little understood despite their substantial bearing on why sexual reproduction is dominant in nature. Here, we examine the role of hybridization in the origin and spread of obligate asexuality in Daphnia pulex, arguably the best-documented case of contagious asexuality. Obligately parthenogenetic (OP) clones of D. pulex have traditionally been separated into 'hybrid' (Ldh SF) and 'nonhybrid' (Ldh SS) forms because the lactase dehydrogenase (Ldh) locus distinguishes the cyclically parthenogenetic (CP) lake dwelling Daphnia pulicaria (Ldh FF) from its ephemeral pond dwelling sister species D. pulex (Ldh SS). The results of our population genetic analyses based on microsatellite loci suggest that both Ldh SS and SF OP individuals can originate from the crossing of CP female F 1 (D. pulex × D. pulicaria) and backcross with males from OP lineages carrying genes that suppress meiosis specifically in female offspring. In previous studies, a suite of diagnostic markers was found to be associated with OP in Ldh SS D. pulex lineages. Our association mapping supports a similar genetic mechanism for the spread of obligate parthenogenesis in Ldh SF OP individuals. Interestingly, our study shows that CP D. pulicaria carry many of the diagnostic microsatellite alleles associated with obligate parthenogenesis. We argue that the assemblage of mutations that suppress meiosis and underlie obligate parthenogenesis in D. pulex originated due to a unique historical hybridization and introgression event between D. pulex and D. pulicaria.

KW - asexuality

KW - Daphnia pulex

KW - Daphnia pulicaria

KW - hybridization

KW - meiosis suppression

KW - parthenogenesis

UR - http://www.scopus.com/inward/record.url?scp=84883214655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883214655&partnerID=8YFLogxK

U2 - 10.1111/mec.12407

DO - 10.1111/mec.12407

M3 - Article

VL - 22

SP - 4549

EP - 4561

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 17

ER -