Abstract

Employing an internal circulation baffled biofilm reactor (ICBBR), we evaluated the mechanisms by which photolysis accelerated the biodegradation and mineralization of pyridine (C<inf>5</inf>H<inf>5</inf>N), a nitrogen-containing heterocyclic compound. We tested the hypothesis that pyridine oxidation is accelerated because a key photolysis intermediate, succinate, is as electron donor that promotes the initial mono-oxygenation of pyridine. Experimentally, longer photolysis time generated more electron-donor products (succinate), which stimulated faster pyridine biodegradation. This pattern was confirmed by directly adding succinate, and the stimulation effect occurred similarly with addition of the same equivalents of acetate and formate. Succinate, whether generated by UV photolysis or added directly, also accelerated mono-oxygenation of the first biodegradation intermediate, 2-hydroxyl pyridine (2HP). 2HP and pyridine were mutually inhibitory in that their mono-oxygenations competed for internal electron donor; thus, the addition of any readily biodegradable donor accelerated both mono-oxygenation steps, as well as mineralization.

Original languageEnglish (US)
Pages (from-to)1792-1800
Number of pages9
JournalBiotechnology and Bioengineering
Volume112
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Photolysis
Biodegradation
Pyridine
Electrons
Oxygenation
Succinic Acid
formic acid
Hydroxyl Radical
Heterocyclic Compounds
Biofilms
pyridine
Acetates
Nitrogen
Oxidation

Keywords

  • Electron donors
  • Mono-oxygenation reaction
  • Pyridine

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

The role of electron donors generated from UV photolysis for accelerating pyridine biodegradation. / Tang, Yingxia; Zhang, Yongming; Yan, Ning; Liu, Rui; Rittmann, Bruce.

In: Biotechnology and Bioengineering, Vol. 112, No. 9, 01.09.2015, p. 1792-1800.

Research output: Contribution to journalArticle

Tang, Yingxia ; Zhang, Yongming ; Yan, Ning ; Liu, Rui ; Rittmann, Bruce. / The role of electron donors generated from UV photolysis for accelerating pyridine biodegradation. In: Biotechnology and Bioengineering. 2015 ; Vol. 112, No. 9. pp. 1792-1800.
@article{c65f9e1484d3443bb1d69af7255b238f,
title = "The role of electron donors generated from UV photolysis for accelerating pyridine biodegradation",
abstract = "Employing an internal circulation baffled biofilm reactor (ICBBR), we evaluated the mechanisms by which photolysis accelerated the biodegradation and mineralization of pyridine (C5H5N), a nitrogen-containing heterocyclic compound. We tested the hypothesis that pyridine oxidation is accelerated because a key photolysis intermediate, succinate, is as electron donor that promotes the initial mono-oxygenation of pyridine. Experimentally, longer photolysis time generated more electron-donor products (succinate), which stimulated faster pyridine biodegradation. This pattern was confirmed by directly adding succinate, and the stimulation effect occurred similarly with addition of the same equivalents of acetate and formate. Succinate, whether generated by UV photolysis or added directly, also accelerated mono-oxygenation of the first biodegradation intermediate, 2-hydroxyl pyridine (2HP). 2HP and pyridine were mutually inhibitory in that their mono-oxygenations competed for internal electron donor; thus, the addition of any readily biodegradable donor accelerated both mono-oxygenation steps, as well as mineralization.",
keywords = "Electron donors, Mono-oxygenation reaction, Pyridine",
author = "Yingxia Tang and Yongming Zhang and Ning Yan and Rui Liu and Bruce Rittmann",
year = "2015",
month = "9",
day = "1",
doi = "10.1002/bit.25605",
language = "English (US)",
volume = "112",
pages = "1792--1800",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - The role of electron donors generated from UV photolysis for accelerating pyridine biodegradation

AU - Tang, Yingxia

AU - Zhang, Yongming

AU - Yan, Ning

AU - Liu, Rui

AU - Rittmann, Bruce

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Employing an internal circulation baffled biofilm reactor (ICBBR), we evaluated the mechanisms by which photolysis accelerated the biodegradation and mineralization of pyridine (C5H5N), a nitrogen-containing heterocyclic compound. We tested the hypothesis that pyridine oxidation is accelerated because a key photolysis intermediate, succinate, is as electron donor that promotes the initial mono-oxygenation of pyridine. Experimentally, longer photolysis time generated more electron-donor products (succinate), which stimulated faster pyridine biodegradation. This pattern was confirmed by directly adding succinate, and the stimulation effect occurred similarly with addition of the same equivalents of acetate and formate. Succinate, whether generated by UV photolysis or added directly, also accelerated mono-oxygenation of the first biodegradation intermediate, 2-hydroxyl pyridine (2HP). 2HP and pyridine were mutually inhibitory in that their mono-oxygenations competed for internal electron donor; thus, the addition of any readily biodegradable donor accelerated both mono-oxygenation steps, as well as mineralization.

AB - Employing an internal circulation baffled biofilm reactor (ICBBR), we evaluated the mechanisms by which photolysis accelerated the biodegradation and mineralization of pyridine (C5H5N), a nitrogen-containing heterocyclic compound. We tested the hypothesis that pyridine oxidation is accelerated because a key photolysis intermediate, succinate, is as electron donor that promotes the initial mono-oxygenation of pyridine. Experimentally, longer photolysis time generated more electron-donor products (succinate), which stimulated faster pyridine biodegradation. This pattern was confirmed by directly adding succinate, and the stimulation effect occurred similarly with addition of the same equivalents of acetate and formate. Succinate, whether generated by UV photolysis or added directly, also accelerated mono-oxygenation of the first biodegradation intermediate, 2-hydroxyl pyridine (2HP). 2HP and pyridine were mutually inhibitory in that their mono-oxygenations competed for internal electron donor; thus, the addition of any readily biodegradable donor accelerated both mono-oxygenation steps, as well as mineralization.

KW - Electron donors

KW - Mono-oxygenation reaction

KW - Pyridine

UR - http://www.scopus.com/inward/record.url?scp=84937738950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937738950&partnerID=8YFLogxK

U2 - 10.1002/bit.25605

DO - 10.1002/bit.25605

M3 - Article

C2 - 25854706

AN - SCOPUS:84937738950

VL - 112

SP - 1792

EP - 1800

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 9

ER -