The RNA subunit of ribonuclease P from the zebrafish, Danio rerio

Paul S. Eder, Ashok Srinivasan, Mark C. Fishman, Sidney Altman

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A simple strategy has been devised to identify the gene encoding the RNA subunit of RNase P from the zebrafish, Danio rerio. The sequence obtained by amplification of genomic DNA with primers based on sequences common to two other vertebrates was confirmed by reverse transcription and amplification of RNA from a partially purified preparation of the holoenzyme. The 5' and 3' ends were determined by cyclizing the RNA, followed by reverse transcription and sequencing across the ligated RNA junction. The zebrafish sequence is 63% identical to that of Xenopus laevis nuclear RNase P RNA and 69% identical to the human RNase P RNA. A consensus secondary structure was constructed based on these nucleotide identities and on the many compensatory base changes in several regions among these three RNAs. The strategy used to obtain the zebrafish sequence should be useful in deriving analogous gene sequences from diverse classes of eukaryotes.

Original languageEnglish (US)
Pages (from-to)21031-21036
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number35
DOIs
StatePublished - Sep 16 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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