The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme

Cecilia Guerrier-Takada, Katheleen Gardiner, Terry Marsh, Norman Pace, Sidney Altman

Research output: Contribution to journalArticle

1792 Citations (Scopus)

Abstract

The RNA moieties of ribonuclease P purified from both E. coli (M1 RNA) and B. subtilis (P-RNA) can cleave tRNA precursor molecules in buffers containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine. The RNA acts as a true catalyst under these conditions whereas the protein moieties of the enzymes alone show no catalytic activity. However, in buffers containing 5-10 mM Mg2+ (in the absence of spermidine) both kinds of subunits are required for enzymatic activity, as shown previously. In the presence of low concentrations of Mg2+, in vitro, the RNA and protein subunits from one species can complement subunits from the other species in reconstitution experiments. When the precursor to E. coli 4.5S RNA is used as a substrate, only the enzyme complexes formed with M1 RNA from E. coli and the protein moieties from either bacterial species are active.

Original languageEnglish (US)
Pages (from-to)849-857
Number of pages9
JournalCell
Volume35
Issue number3 PART 2
DOIs
StatePublished - 1983
Externally publishedYes

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Ribonuclease P
Catalytic Domain
RNA
Spermidine
Enzymes
Buffers
Escherichia coli Proteins
RNA Precursors
Protein Subunits
Escherichia coli
Catalyst activity
Catalysts
Molecules
Substrates
Proteins
Experiments

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N., & Altman, S. (1983). The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell, 35(3 PART 2), 849-857. https://doi.org/10.1016/0092-8674(83)90117-4

The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. / Guerrier-Takada, Cecilia; Gardiner, Katheleen; Marsh, Terry; Pace, Norman; Altman, Sidney.

In: Cell, Vol. 35, No. 3 PART 2, 1983, p. 849-857.

Research output: Contribution to journalArticle

Guerrier-Takada, C, Gardiner, K, Marsh, T, Pace, N & Altman, S 1983, 'The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme', Cell, vol. 35, no. 3 PART 2, pp. 849-857. https://doi.org/10.1016/0092-8674(83)90117-4
Guerrier-Takada, Cecilia ; Gardiner, Katheleen ; Marsh, Terry ; Pace, Norman ; Altman, Sidney. / The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. In: Cell. 1983 ; Vol. 35, No. 3 PART 2. pp. 849-857.
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