The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya

The mtDNA perspective

T. Lehmann, C. R. Blackston, N. J. Besansky, A. A. Escalante, F. H. Collins, W. A. Hawley

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Descriptions of A. gambiae population structure based on microsatellite loci and mitochondrial DNA (mtDNA) were incongruent. High differentiation of populations was measured across the Rift Valley by microsatellites, but no differentiation was detected based on mtDNA. To resolve this conflict, we compared the old data to new mtDNA data using the same specimen previously genotyped in microsatellite loci. Analysis of a larger number of mtDNA sequences resulted in high and significant differentiation between populations across the Rift Valley. We developed a method to assess whether differentiation across the Rift Valley was generated by pure drift rather than mutation-drift, based on DNA sequence data. Applying this method to the mtDNA data suggested that pure drift was the primary force generating differentiation between the populations across the Rift, while mutation-drift generated differentiation across the continent. Given adequate sample size, mtDNA provided congruent results with microsatellite loci.

Original languageEnglish (US)
Pages (from-to)165-168
Number of pages4
JournalJournal of Heredity
Volume91
Issue number2
StatePublished - Mar 2000
Externally publishedYes

Fingerprint

Anopheles gambiae
Gene Flow
Kenya
Mitochondrial DNA
gene flow
mitochondrial DNA
valleys
Microsatellite Repeats
microsatellite repeats
Population
loci
mutation
nucleotide sequences
Mutation
Sample Size
population structure
methodology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)

Cite this

Lehmann, T., Blackston, C. R., Besansky, N. J., Escalante, A. A., Collins, F. H., & Hawley, W. A. (2000). The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya: The mtDNA perspective. Journal of Heredity, 91(2), 165-168.

The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya : The mtDNA perspective. / Lehmann, T.; Blackston, C. R.; Besansky, N. J.; Escalante, A. A.; Collins, F. H.; Hawley, W. A.

In: Journal of Heredity, Vol. 91, No. 2, 03.2000, p. 165-168.

Research output: Contribution to journalArticle

Lehmann, T, Blackston, CR, Besansky, NJ, Escalante, AA, Collins, FH & Hawley, WA 2000, 'The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya: The mtDNA perspective', Journal of Heredity, vol. 91, no. 2, pp. 165-168.
Lehmann T, Blackston CR, Besansky NJ, Escalante AA, Collins FH, Hawley WA. The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya: The mtDNA perspective. Journal of Heredity. 2000 Mar;91(2):165-168.
Lehmann, T. ; Blackston, C. R. ; Besansky, N. J. ; Escalante, A. A. ; Collins, F. H. ; Hawley, W. A. / The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya : The mtDNA perspective. In: Journal of Heredity. 2000 ; Vol. 91, No. 2. pp. 165-168.
@article{e86f06afae9e44de84ff1c0e1c7c86a0,
title = "The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya: The mtDNA perspective",
abstract = "Descriptions of A. gambiae population structure based on microsatellite loci and mitochondrial DNA (mtDNA) were incongruent. High differentiation of populations was measured across the Rift Valley by microsatellites, but no differentiation was detected based on mtDNA. To resolve this conflict, we compared the old data to new mtDNA data using the same specimen previously genotyped in microsatellite loci. Analysis of a larger number of mtDNA sequences resulted in high and significant differentiation between populations across the Rift Valley. We developed a method to assess whether differentiation across the Rift Valley was generated by pure drift rather than mutation-drift, based on DNA sequence data. Applying this method to the mtDNA data suggested that pure drift was the primary force generating differentiation between the populations across the Rift, while mutation-drift generated differentiation across the continent. Given adequate sample size, mtDNA provided congruent results with microsatellite loci.",
author = "T. Lehmann and Blackston, {C. R.} and Besansky, {N. J.} and Escalante, {A. A.} and Collins, {F. H.} and Hawley, {W. A.}",
year = "2000",
month = "3",
language = "English (US)",
volume = "91",
pages = "165--168",
journal = "Journal of Heredity",
issn = "0022-1503",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - The Rift Valley complex as a barrier to gene flow for Anopheles gambiae in Kenya

T2 - The mtDNA perspective

AU - Lehmann, T.

AU - Blackston, C. R.

AU - Besansky, N. J.

AU - Escalante, A. A.

AU - Collins, F. H.

AU - Hawley, W. A.

PY - 2000/3

Y1 - 2000/3

N2 - Descriptions of A. gambiae population structure based on microsatellite loci and mitochondrial DNA (mtDNA) were incongruent. High differentiation of populations was measured across the Rift Valley by microsatellites, but no differentiation was detected based on mtDNA. To resolve this conflict, we compared the old data to new mtDNA data using the same specimen previously genotyped in microsatellite loci. Analysis of a larger number of mtDNA sequences resulted in high and significant differentiation between populations across the Rift Valley. We developed a method to assess whether differentiation across the Rift Valley was generated by pure drift rather than mutation-drift, based on DNA sequence data. Applying this method to the mtDNA data suggested that pure drift was the primary force generating differentiation between the populations across the Rift, while mutation-drift generated differentiation across the continent. Given adequate sample size, mtDNA provided congruent results with microsatellite loci.

AB - Descriptions of A. gambiae population structure based on microsatellite loci and mitochondrial DNA (mtDNA) were incongruent. High differentiation of populations was measured across the Rift Valley by microsatellites, but no differentiation was detected based on mtDNA. To resolve this conflict, we compared the old data to new mtDNA data using the same specimen previously genotyped in microsatellite loci. Analysis of a larger number of mtDNA sequences resulted in high and significant differentiation between populations across the Rift Valley. We developed a method to assess whether differentiation across the Rift Valley was generated by pure drift rather than mutation-drift, based on DNA sequence data. Applying this method to the mtDNA data suggested that pure drift was the primary force generating differentiation between the populations across the Rift, while mutation-drift generated differentiation across the continent. Given adequate sample size, mtDNA provided congruent results with microsatellite loci.

UR - http://www.scopus.com/inward/record.url?scp=0034009843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034009843&partnerID=8YFLogxK

M3 - Article

VL - 91

SP - 165

EP - 168

JO - Journal of Heredity

JF - Journal of Heredity

SN - 0022-1503

IS - 2

ER -