The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii

Stefano Santabarbara, Anna Paola Casazza, Kulsam Ali, Chloe K. Economou, Thanyanun Wannathong, Francesca Zito, Kevin Redding, Fabrice Rappaport, Saul Purton

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b6f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b6f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.

    Original languageEnglish (US)
    Pages (from-to)535-546
    Number of pages12
    JournalPlant Physiology
    Volume161
    Issue number1
    DOIs
    StatePublished - Jan 2013

    Fingerprint

    Photosynthetic Reaction Center Complex Proteins
    Chlamydomonas reinhardtii
    Carotenoids
    carotenoids
    Cytochrome b6f Complex
    cytochromes
    phytoene synthase
    mutants
    xanthophylls
    light harvesting complex
    Xanthophylls
    photosystem I
    Photosystem I Protein Complex
    carotenes
    Chlorophyta
    Photosystem II Protein Complex
    photosystem II
    electron transfer
    functional properties
    biosynthesis

    ASJC Scopus subject areas

    • Plant Science
    • Genetics
    • Physiology

    Cite this

    Santabarbara, S., Casazza, A. P., Ali, K., Economou, C. K., Wannathong, T., Zito, F., ... Purton, S. (2013). The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. Plant Physiology, 161(1), 535-546. https://doi.org/10.1104/pp.112.205260

    The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. / Santabarbara, Stefano; Casazza, Anna Paola; Ali, Kulsam; Economou, Chloe K.; Wannathong, Thanyanun; Zito, Francesca; Redding, Kevin; Rappaport, Fabrice; Purton, Saul.

    In: Plant Physiology, Vol. 161, No. 1, 01.2013, p. 535-546.

    Research output: Contribution to journalArticle

    Santabarbara, S, Casazza, AP, Ali, K, Economou, CK, Wannathong, T, Zito, F, Redding, K, Rappaport, F & Purton, S 2013, 'The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii' Plant Physiology, vol. 161, no. 1, pp. 535-546. https://doi.org/10.1104/pp.112.205260
    Santabarbara, Stefano ; Casazza, Anna Paola ; Ali, Kulsam ; Economou, Chloe K. ; Wannathong, Thanyanun ; Zito, Francesca ; Redding, Kevin ; Rappaport, Fabrice ; Purton, Saul. / The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. In: Plant Physiology. 2013 ; Vol. 161, No. 1. pp. 535-546.
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