The rate and molecular spectrum of spontaneous mutations in arabidopsis thaliana

Stephan Ossowski, Korbinian Schneeberger, José Ignacio Lucas-Lledó, Norman Warthmann, Richard M. Clark, Ruth G. Shaw, Detlef Weigel, Michael Lynch

Research output: Contribution to journalArticle

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Abstract

To take complete advantage of information on within-species polymorphism and divergence from close relatives, one needs to know the rate and the molecular spectrum of spontaneous mutations. To this end, we have searched for de novo spontaneous mutations in the complete nuclear genomes of five Arabidopsis thaliana mutation accumulation lines that had been maintained by single-seed descent for 30 generations. We identified and validated 99 base substitutions and 17 small and large insertions and deletions. Our results imply a spontaneous mutation rate of 7 × 10-9 base substitutions per site per generation, the majority of which are G:C→A:T transitions. We explain this very biased spectrum of base substitution mutations as a result of two main processes: deamination of methylated cytosines and ultraviolet light-induced mutagenesis.

Original languageEnglish (US)
Pages (from-to)92-94
Number of pages3
JournalScience
Volume327
Issue number5961
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

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Arabidopsis
Mutation
Deamination
Cytosine
Mutation Rate
Ultraviolet Rays
Mutagenesis
Seeds
Genome

ASJC Scopus subject areas

  • General

Cite this

Ossowski, S., Schneeberger, K., Lucas-Lledó, J. I., Warthmann, N., Clark, R. M., Shaw, R. G., ... Lynch, M. (2010). The rate and molecular spectrum of spontaneous mutations in arabidopsis thaliana. Science, 327(5961), 92-94. https://doi.org/10.1126/science.1180677

The rate and molecular spectrum of spontaneous mutations in arabidopsis thaliana. / Ossowski, Stephan; Schneeberger, Korbinian; Lucas-Lledó, José Ignacio; Warthmann, Norman; Clark, Richard M.; Shaw, Ruth G.; Weigel, Detlef; Lynch, Michael.

In: Science, Vol. 327, No. 5961, 01.01.2010, p. 92-94.

Research output: Contribution to journalArticle

Ossowski, S, Schneeberger, K, Lucas-Lledó, JI, Warthmann, N, Clark, RM, Shaw, RG, Weigel, D & Lynch, M 2010, 'The rate and molecular spectrum of spontaneous mutations in arabidopsis thaliana', Science, vol. 327, no. 5961, pp. 92-94. https://doi.org/10.1126/science.1180677
Ossowski S, Schneeberger K, Lucas-Lledó JI, Warthmann N, Clark RM, Shaw RG et al. The rate and molecular spectrum of spontaneous mutations in arabidopsis thaliana. Science. 2010 Jan 1;327(5961):92-94. https://doi.org/10.1126/science.1180677
Ossowski, Stephan ; Schneeberger, Korbinian ; Lucas-Lledó, José Ignacio ; Warthmann, Norman ; Clark, Richard M. ; Shaw, Ruth G. ; Weigel, Detlef ; Lynch, Michael. / The rate and molecular spectrum of spontaneous mutations in arabidopsis thaliana. In: Science. 2010 ; Vol. 327, No. 5961. pp. 92-94.
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