The psbC start codon in Synechocystis sp. PCC 6803

Shelly D. Carpenter, Jeroen Charite, Beth Eggers, Willem Vermaas

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The translation start codon for psbC, the gene encoding CP43, a chlorophyll-binding protein of photosystem II, has been identified for the cyanobacterium Synechosystis sp. PCC 6803 using site-directed mutagenesis. An AUG codon, about 50 bases upstream from the end of psbD-I had previously been assumed to be the translation start site of psbC. However, the fact that the AUG codon is not present in psbC from several other organisms, whereas a GUG codon 14 bases upstream from the end of psbD-I is strictly conserved suggests that CP43 translation starts at the latter codon. Mutation of GUG, but not of AUG, led to a loss of CP43 and photoautotrophic growth, indicating that the GUG codon is the sole initiation site for translation of the CP43 protein in Synechocystis sp. PCC 6803.

Original languageEnglish (US)
Pages (from-to)135-137
Number of pages3
JournalFEBS Letters
Volume260
Issue number1
DOIs
StatePublished - Jan 15 1990

Fingerprint

Chlorophyll Binding Proteins
Synechocystis
Mutagenesis
Gene encoding
Photosystem II Protein Complex
Initiator Codon
Codon
Proteins
Cyanobacteria
Protein Biosynthesis
Site-Directed Mutagenesis
Mutation
Growth
Genes

Keywords

  • CP43
  • Cyanobacterium
  • Gene, psbC
  • Photosystem II
  • Translation initiation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The psbC start codon in Synechocystis sp. PCC 6803. / Carpenter, Shelly D.; Charite, Jeroen; Eggers, Beth; Vermaas, Willem.

In: FEBS Letters, Vol. 260, No. 1, 15.01.1990, p. 135-137.

Research output: Contribution to journalArticle

Carpenter, Shelly D. ; Charite, Jeroen ; Eggers, Beth ; Vermaas, Willem. / The psbC start codon in Synechocystis sp. PCC 6803. In: FEBS Letters. 1990 ; Vol. 260, No. 1. pp. 135-137.
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AB - The translation start codon for psbC, the gene encoding CP43, a chlorophyll-binding protein of photosystem II, has been identified for the cyanobacterium Synechosystis sp. PCC 6803 using site-directed mutagenesis. An AUG codon, about 50 bases upstream from the end of psbD-I had previously been assumed to be the translation start site of psbC. However, the fact that the AUG codon is not present in psbC from several other organisms, whereas a GUG codon 14 bases upstream from the end of psbD-I is strictly conserved suggests that CP43 translation starts at the latter codon. Mutation of GUG, but not of AUG, led to a loss of CP43 and photoautotrophic growth, indicating that the GUG codon is the sole initiation site for translation of the CP43 protein in Synechocystis sp. PCC 6803.

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