The potential and challenges of nanopore sequencing

Daniel Branton, David W. Deamer, Andre Marziali, Hagan Bayley, Steven A. Benner, Thomas Butler, Massimiliano Di Ventra, Slaven Garaj, Andrew Hibbs, Xiaohua Huang, Stevan B. Jovanovich, Stuart Lindsay, Predrag S. Krstic, Xinshen Sean Ling, Carlos H. Mastrangelo, Amit Meller, John S. Oliver, Yuriy V. Pershin, Robert Riehn, Matthew WigginJeffery A. Schloss, Vincent Tabard-Cossa, Gautam V. Soni, Meni Wanunu

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of third generation' instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.

Original languageEnglish (US)
Title of host publicationNanoscience and Technology: A Collection of Reviews from Nature Journals
PublisherWorld Scientific Publishing Co.
Pages261-268
Number of pages8
ISBN (Electronic)9789814287005
ISBN (Print)9814282685, 9789814282680
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'The potential and challenges of nanopore sequencing'. Together they form a unique fingerprint.

  • Cite this

    Branton, D., Deamer, D. W., Marziali, A., Bayley, H., Benner, S. A., Butler, T., Ventra, M. D., Garaj, S., Hibbs, A., Huang, X., Jovanovich, S. B., Lindsay, S., Krstic, P. S., Ling, X. S., Mastrangelo, C. H., Meller, A., Oliver, J. S., Pershin, Y. V., Riehn, R., ... Wanunu, M. (2009). The potential and challenges of nanopore sequencing. In Nanoscience and Technology: A Collection of Reviews from Nature Journals (pp. 261-268). World Scientific Publishing Co.. https://doi.org/10.1142/9789814287005_0027