The potential and challenges of nanopore sequencing

Daniel Branton, David W. Deamer, Andre Marziali, Hagan Bayley, Steven A. Benner, Thomas Butler, Massimiliano Di Ventra, Slaven Garaj, Andrew Hibbs, Xiaohua Huang, Stevan B. Jovanovich, Predrag S. Krstic, Stuart Lindsay, Xinsheng Sean Ling, Carlos H. Mastrangelo, Amit Meller, John S. Oliver, Yuriy V. Pershin, J. Michael Ramsey, Robert RiehnGautam V. Soni, Vincent Tabard-Cossa, Meni Wanunu, Matthew Wiggin, Jeffery A. Schloss

Research output: Contribution to journalReview article

1643 Scopus citations

Abstract

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for ∼$1,000 in ∼24 h.

Original languageEnglish (US)
Pages (from-to)1146-1153
Number of pages8
JournalNature biotechnology
Volume26
Issue number10
DOIs
StatePublished - Oct 2008

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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    Branton, D., Deamer, D. W., Marziali, A., Bayley, H., Benner, S. A., Butler, T., Di Ventra, M., Garaj, S., Hibbs, A., Huang, X., Jovanovich, S. B., Krstic, P. S., Lindsay, S., Ling, X. S., Mastrangelo, C. H., Meller, A., Oliver, J. S., Pershin, Y. V., Ramsey, J. M., ... Schloss, J. A. (2008). The potential and challenges of nanopore sequencing. Nature biotechnology, 26(10), 1146-1153. https://doi.org/10.1038/nbt.1495