The nuclear vitamin D receptor: From clinical radioreceptor assay of the vitamin D hormone to genomics, proteomics and a novel ligand

Mark R. Haussler, Carol A. Haussler, Peter Jurutka, Carlos Encinas Dominguez, Jui Cheng Hsieh, Michelle L. Thatcher, G. Kerr Whitfield

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Vitamin D is bioactivated in kidney to its hormonal form, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), functioning to prevent rickets/osteomalacia by stimulating small intestinal calcium absorption. Plasma 1,25(OH)2D3 is depressed in patients with chronic renal failure and elevated in subjects with calcium urolithiasis, but normal in osteoporosis. The chromosomal gene encoding the nuclear vitamin D receptor (VDR) contains two common polymorphisms that influence VDR functional activity, which encompasses 1,25 (OH)2D3-ligand-dependent heterodimerization with retinoid X receptor (RXR) and recruitment of cell-specific coactivators for enhancement of target gene transcription. A newly discovered ligand for VDR is lithocholic acid (LCA), a secondary bile acid that is a causative agent in colon cancer promoted by high fat diets. LCA activates the VDR-RXR complex in colon to stimulate the transcription of cytochrome P450 3A4, which detoxifies LCA. This process is potentiated by 1,25(OH)2D3 binding to VDR, explaining mechanistically the epidemiologic finding that vitamin D prevents colon cancer.

Original languageEnglish (US)
Pages (from-to)221-228
Number of pages8
JournalJournal of Clinical Ligand Assay
Volume25
Issue number2
StatePublished - Jun 2002
Externally publishedYes

Fingerprint

Radioligand Assay
Calcitriol Receptors
Genomics
Vitamin D
Proteomics
Lithocholic Acid
Assays
Hormones
Ligands
Retinoid X Receptors
Transcription
Colonic Neoplasms
Calcium
Cytochrome P-450 CYP3A
Osteomalacia
Rickets
Gene encoding
Urolithiasis
Calcitriol
Intestinal Absorption

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology

Cite this

Haussler, M. R., Haussler, C. A., Jurutka, P., Encinas Dominguez, C., Hsieh, J. C., Thatcher, M. L., & Whitfield, G. K. (2002). The nuclear vitamin D receptor: From clinical radioreceptor assay of the vitamin D hormone to genomics, proteomics and a novel ligand. Journal of Clinical Ligand Assay, 25(2), 221-228.

The nuclear vitamin D receptor : From clinical radioreceptor assay of the vitamin D hormone to genomics, proteomics and a novel ligand. / Haussler, Mark R.; Haussler, Carol A.; Jurutka, Peter; Encinas Dominguez, Carlos; Hsieh, Jui Cheng; Thatcher, Michelle L.; Whitfield, G. Kerr.

In: Journal of Clinical Ligand Assay, Vol. 25, No. 2, 06.2002, p. 221-228.

Research output: Contribution to journalArticle

Haussler, MR, Haussler, CA, Jurutka, P, Encinas Dominguez, C, Hsieh, JC, Thatcher, ML & Whitfield, GK 2002, 'The nuclear vitamin D receptor: From clinical radioreceptor assay of the vitamin D hormone to genomics, proteomics and a novel ligand', Journal of Clinical Ligand Assay, vol. 25, no. 2, pp. 221-228.
Haussler, Mark R. ; Haussler, Carol A. ; Jurutka, Peter ; Encinas Dominguez, Carlos ; Hsieh, Jui Cheng ; Thatcher, Michelle L. ; Whitfield, G. Kerr. / The nuclear vitamin D receptor : From clinical radioreceptor assay of the vitamin D hormone to genomics, proteomics and a novel ligand. In: Journal of Clinical Ligand Assay. 2002 ; Vol. 25, No. 2. pp. 221-228.
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