The next generation of proteomic nanochips in biomarker discovery

Ye Hu, Ali Bouamrani, Xuewu Liu, Ennio Tasciotti, Li Li, Mauro Ferrari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

As a potential source of diagnostic biomarkers for diseases at their early stage, the low-molecular weight (LMW) region of the blood proteome has gained increased interest recently. However, the presence of highly abundant proteins and the large dynamic range of serum/plasma proteins ultimately limit the sensitivity of the detection of low abundant species. In this study, we present a novel size-exclusion strategy based on nanoporous silica chips for the efficient removal of the high molecular weight proteins and for the specific isolation and enrichment of LMW species present in biological complex. We applied the Nanoporous Silica Chip Technology at the analysis of complex proteomic samples such as human serum and developed proteomic nanochips with different nanophase characteristics to specifically target the low molecular weight species present in the human circulating peptidome. Harvested peptides were analysed by MALDI-TOF and profiles consisting of more than 300 peaks in the range 800-20,000 m/z were generated. Tunable pore sizes, pore structure and surface chemistries were used as integrated "processors" for the recovery of LMW peptides and proteins. This approach will help in the selection of individualized therapeutic combinations that target the entire cancer-specific protein network, in the real-time assessment of therapeutic efficacy and toxicity, and in the rational modulation of therapy based on changes in the cancer protein network associated with prognosis and drug resistance.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Pages238-241
Number of pages4
StatePublished - Dec 1 2009
EventNanotechnology 2009: Biofuels, Renewable Energy, Coatings, Fluidics and Compact Modeling - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States
Duration: May 3 2009May 7 2009

Publication series

NameTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Volume3

Other

OtherNanotechnology 2009: Biofuels, Renewable Energy, Coatings, Fluidics and Compact Modeling - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
CountryUnited States
CityHouston, TX
Period5/3/095/7/09

Keywords

  • Low molecular weight proteome
  • Nanoporous silica thin film

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials

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  • Cite this

    Hu, Y., Bouamrani, A., Liu, X., Tasciotti, E., Li, L., & Ferrari, M. (2009). The next generation of proteomic nanochips in biomarker discovery. In Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 (pp. 238-241). (Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009; Vol. 3).