The Neurospora crassa exocyst complex tethers Spitzenkörper vesicles to the apical plasma membrane during polarized growth

Meritxell Riquelme, Erin L. Bredeweg, Olga Callejas-Negrete, Robert Roberson, Sarah Ludwig, Alejandro Beltrán-Aguilar, Stephan Seiler, Peter Novick, Michael Freitag

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Abstract

Fungal hyphae are among the most highly polarized cells. Hyphal polarized growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex is required for tethering of secretory vesicles to the apical plasma membrane. We determined that the presence of an octameric exocyst complex is required for the formation of a functional Spitzenkörper and maintenance of regular hyphal growth in Neurospora crassa. Two distinct localization patterns of exocyst subunits at the hyphal tip suggest the dynamic formation of two assemblies. The EXO-70/EXO-84 subunits are found at the peripheral part of the Spitzenkörper, which partially coincides with the outer macrovesicular layer, whereas exocyst components SEC-5, -6, -8, and -15 form a delimited crescent at the apical plasma membrane. Localization of SEC-6 and EXO-70 to the plasma membrane and the Spitzenkörper, respectively, depends on actin and microtubule cytoskeletons. The apical region of exocyst-mediated vesicle fusion, elucidated by the plasma membrane-associated exocyst subunits, indicates the presence of an exocytotic gradient with a tip-high maximum that dissipates gradually toward the subapex, confirming the earlier predictions of the vesicle supply center model for hyphal morphogenesis.

Original languageEnglish (US)
Pages (from-to)1312-1326
Number of pages15
JournalMolecular biology of the cell
Volume25
Issue number8
DOIs
StatePublished - Apr 15 2014

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Riquelme, M., Bredeweg, E. L., Callejas-Negrete, O., Roberson, R., Ludwig, S., Beltrán-Aguilar, A., Seiler, S., Novick, P., & Freitag, M. (2014). The Neurospora crassa exocyst complex tethers Spitzenkörper vesicles to the apical plasma membrane during polarized growth. Molecular biology of the cell, 25(8), 1312-1326. https://doi.org/10.1091/mbc.E13-06-0299