The Mycobacterium tuberculosis relBE toxin

antitoxin genes are stress-responsive modules that regulate growth through translation inhibition

Shaleen B. Korch, Vandana Malhotra, Heidi Contreras, Josephine E. Clark-Curtiss

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Toxin-antitoxin (TA) genes are ubiquitous among bacteria and are associated with persistence and dormancy. Following exposure to unfavorable environmental stimuli, several species (Escherichia coli, Staphylococcus aureus, Myxococcus xanthus) employ toxin proteins such as RelE and MazF to downregulate growth or initiate cell death. Mycobacterium tuberculosis possesses three Rel TA modules (RelMtb): RelBEMtb, RelFGMtb and RelJKMtb (Rv1246c-Rv1247c, Rv2865-Rv2866, and Rv3357-Rv3358, respectively), which inhibit mycobacterial growth when the toxin gene (relE, relG, relK) is expressed independently of the antitoxin gene (relB, relF, relJ). In the present study, we examined the in vivo mechanism of the RelEMtb toxin protein, the impact of RelEMtb on M. tuberculosis physiology and the environmental conditions that regulate all three relMtb modules. RelEMtb negatively impacts growth and the structural integrity of the mycobacterial envelope, generating cells with aberrant forms that are prone to extensive aggregation. At a time coincident with growth defects, RelEMtb mediates mRNA degradation in vivo resulting in significant changes to the proteome. We establish that relMtb modules are stress responsive, as all three operons are transcriptionally activated following mycobacterial exposure to oxidative stress or nitrogen-limiting growth environments. Here we present evidence that the relMtb toxin:antitoxin family is stress-responsive and, through the degradation of mRNA, the RelEMtb toxin influences the growth, proteome and morphology of mycobacterial cells.

Original languageEnglish (US)
Pages (from-to)783-795
Number of pages13
JournalJournal of Microbiology
Volume53
Issue number11
DOIs
StatePublished - Nov 1 2015

Fingerprint

Antitoxins
Mycobacterium tuberculosis
Growth
Genes
RNA Stability
Proteome
Myxococcus xanthus
Operon
Staphylococcus aureus
Proteins
Oxidative Stress
Cell Death
Nitrogen
Down-Regulation
Escherichia coli
Bacteria

Keywords

  • growth
  • mRNA
  • Mycobacterium tuberculosis
  • relBE
  • stress
  • toxin:antitoxin

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

The Mycobacterium tuberculosis relBE toxin : antitoxin genes are stress-responsive modules that regulate growth through translation inhibition. / Korch, Shaleen B.; Malhotra, Vandana; Contreras, Heidi; Clark-Curtiss, Josephine E.

In: Journal of Microbiology, Vol. 53, No. 11, 01.11.2015, p. 783-795.

Research output: Contribution to journalArticle

Korch, Shaleen B. ; Malhotra, Vandana ; Contreras, Heidi ; Clark-Curtiss, Josephine E. / The Mycobacterium tuberculosis relBE toxin : antitoxin genes are stress-responsive modules that regulate growth through translation inhibition. In: Journal of Microbiology. 2015 ; Vol. 53, No. 11. pp. 783-795.
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