The molecular basis of triazine-herbicide resistance in higher-plant chloroplasts

Joseph Hirschber, Anthony Bleecker, David J. Kyle, Lee McIntosh, Charles J. Arntzen

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Triazine herbicides inhibit photosynthesis by blocking electron transport in photosystem II. The target site ofthe herbicide was identified as a chloroplast thylakoid polypeptide (the QB protein) of 32,000 daltons. Studies of triazine-resistant weed biotypes suggested that a subtle change in the QB protein caused the resistance. We have cloned the chloroplast gene (psbA) that codes this protein from herbicide-resistant and herbicide-susceptible biotypes of Solanum nigrum. By DN A sequencing we detected a single base substitution in the psbA gene of the resistant plants, resulting in an amino acid change (serine to glycine for the susceptible to resistance conversion). This mutation is exactly the same one which we have described in a herbicide-resistant biotype of Amaranthus hybridus.

Original languageEnglish (US)
Pages (from-to)412-420
Number of pages9
JournalZeitschrift fur Naturforschung. C, Journal of biosciences
Volume39
Issue number5
DOIs
StatePublished - May 1 1984
Externally publishedYes

Fingerprint

Herbicide Resistance
Triazines
Herbicides
Chloroplasts
Solanum nigrum
Genes
Chloroplast Genes
Amaranthus
Plant Genes
Proteins
Photosystem II Protein Complex
Photosynthesis
Electron Transport
Glycine
Serine
Substitution reactions
Amino Acids
Mutation

Keywords

  • Chloroplasts
  • Molecular Basis of Herbicide-Resistance
  • Photosynthesis
  • Triazines

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The molecular basis of triazine-herbicide resistance in higher-plant chloroplasts. / Hirschber, Joseph; Bleecker, Anthony; Kyle, David J.; McIntosh, Lee; Arntzen, Charles J.

In: Zeitschrift fur Naturforschung. C, Journal of biosciences, Vol. 39, No. 5, 01.05.1984, p. 412-420.

Research output: Contribution to journalArticle

Hirschber, Joseph ; Bleecker, Anthony ; Kyle, David J. ; McIntosh, Lee ; Arntzen, Charles J. / The molecular basis of triazine-herbicide resistance in higher-plant chloroplasts. In: Zeitschrift fur Naturforschung. C, Journal of biosciences. 1984 ; Vol. 39, No. 5. pp. 412-420.
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