The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes

K. Walder, L. Kerr-Bayles, A. Civitarese, J. Jowett, J. Curran, K. Elliott, J. Trevaskis, N. Bishara, P. Zimmet, L. Mandarino, E. Ravussin, J. Blangero, A. Kissebah, G. R. Collier

    Research output: Contribution to journalArticle

    68 Scopus citations

    Abstract

    Aims/hypothesis: This study aimed to identify genes that are expressed in skeletal muscle, encode proteins with functional significance in mitochondria, and are associated with type 2 diabetes. Methods: We screened for differentially expressed genes in skeletal muscle of Psammomys obesus (Israeli sand rats), and prioritised these on the basis of genomic localisation and bioinformatics analysis for proteins with likely mitochondrial functions. Results: We identified a mitochondrial intramembrane protease, known as presenilins- associated rhomboid-like protein (PSARL) that is associated with insulin resistance and type 2 diabetes. Expression of PSARL was reduced in skeletal muscle of diabetic Psammomys obesus, and restored after exercise training to successfully treat the diabetes. PSARL gene expression in human skeletal muscle was correlated with insulin sensitivity as assessed by glucose disposal during a hyperinsulinaemic-euglycaemic clamp. In 1,031 human subjects, an amino acid substitution (Leu262Val) in PSARL was associated with increased plasma insulin concentration, a key risk factor for diabetes. Furthermore, this variant interacted strongly with age to affect insulin levels, accounting for 5% of the variation in plasma insulin in elderly subjects. Conclusions/interpretation: Variation in PSARL sequence and/or expression may be an important new risk factor for type 2 diabetes and other components of the metabolic syndrome.

    Original languageEnglish (US)
    Pages (from-to)459-468
    Number of pages10
    JournalDiabetologia
    Volume48
    Issue number3
    DOIs
    StatePublished - Mar 1 2005

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    Keywords

    • Association
    • Gene expression
    • Gene-environment interaction
    • Psammomys obesus

    ASJC Scopus subject areas

    • Internal Medicine
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Walder, K., Kerr-Bayles, L., Civitarese, A., Jowett, J., Curran, J., Elliott, K., Trevaskis, J., Bishara, N., Zimmet, P., Mandarino, L., Ravussin, E., Blangero, J., Kissebah, A., & Collier, G. R. (2005). The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes. Diabetologia, 48(3), 459-468. https://doi.org/10.1007/s00125-005-1675-9