The marginal regions of thylakoid membranes: A partial characterization by polyoxyethylene sorbitan monolaurate (Tween 20) solubilization of spinach thylakoids

Andrew Webber, Kathryn A. Platt‐Aloia, Robert L. Heath, William W. Thomson

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The detergent Tween‐20 solubilized preferentially portions of the marginal regions of Spinacea oleracea L. thylakoid membranes and, thus, opened the inside of the grana to the external media. Differential centrifugation. following Tween‐20 solubilization. enabled separate fractions of grana and stromal‐exposed membranes to be isolated. Analysis of Tween‐20 solubilized material, after pelleting all membrane material by centrifugation at 100 000 g, revealed polypeptides associated with the coupling factor (CF1) particles, cytochrome b6/f and photosystem II complexes, suggesting that the marginal membranes contain these proteins. Concomitantly, the 100 000 g pellet was depleted in cytochrome b6/f and P700, determined spectroscopically, Thus. our results reveal the margin to be a distinct membrane region, which does not contain the light‐harvesting centers of photosystem II (LHC II). The implication of these results, in terms of the energetic interaction of components of granal and stromalexposed membrane regions, is discussed.

Original languageEnglish (US)
Pages (from-to)288-297
Number of pages10
JournalPhysiologia Plantarum
Volume72
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

Keywords

  • Chloroplast
  • margins
  • photochemical coupling
  • Spinacea oleracea
  • spinach
  • thylakoids
  • Tween 20
  • ultrastructure

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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