TY - JOUR
T1 - The marginal regions of thylakoid membranes
T2 - A partial characterization by polyoxyethylene sorbitan monolaurate (Tween 20) solubilization of spinach thylakoids
AU - Webber, Andrew
AU - Platt‐Aloia, Kathryn A.
AU - Heath, Robert L.
AU - Thomson, William W.
PY - 1988
Y1 - 1988
N2 - The detergent Tween‐20 solubilized preferentially portions of the marginal regions of Spinacea oleracea L. thylakoid membranes and, thus, opened the inside of the grana to the external media. Differential centrifugation. following Tween‐20 solubilization. enabled separate fractions of grana and stromal‐exposed membranes to be isolated. Analysis of Tween‐20 solubilized material, after pelleting all membrane material by centrifugation at 100 000 g, revealed polypeptides associated with the coupling factor (CF1) particles, cytochrome b6/f and photosystem II complexes, suggesting that the marginal membranes contain these proteins. Concomitantly, the 100 000 g pellet was depleted in cytochrome b6/f and P700, determined spectroscopically, Thus. our results reveal the margin to be a distinct membrane region, which does not contain the light‐harvesting centers of photosystem II (LHC II). The implication of these results, in terms of the energetic interaction of components of granal and stromalexposed membrane regions, is discussed.
AB - The detergent Tween‐20 solubilized preferentially portions of the marginal regions of Spinacea oleracea L. thylakoid membranes and, thus, opened the inside of the grana to the external media. Differential centrifugation. following Tween‐20 solubilization. enabled separate fractions of grana and stromal‐exposed membranes to be isolated. Analysis of Tween‐20 solubilized material, after pelleting all membrane material by centrifugation at 100 000 g, revealed polypeptides associated with the coupling factor (CF1) particles, cytochrome b6/f and photosystem II complexes, suggesting that the marginal membranes contain these proteins. Concomitantly, the 100 000 g pellet was depleted in cytochrome b6/f and P700, determined spectroscopically, Thus. our results reveal the margin to be a distinct membrane region, which does not contain the light‐harvesting centers of photosystem II (LHC II). The implication of these results, in terms of the energetic interaction of components of granal and stromalexposed membrane regions, is discussed.
KW - Chloroplast
KW - margins
KW - photochemical coupling
KW - Spinacea oleracea
KW - spinach
KW - thylakoids
KW - Tween 20
KW - ultrastructure
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U2 - 10.1111/j.1399-3054.1988.tb05835.x
DO - 10.1111/j.1399-3054.1988.tb05835.x
M3 - Article
AN - SCOPUS:85013543663
SN - 0031-9317
VL - 72
SP - 288
EP - 297
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 2
ER -