The Making of a Queen: TOR Pathway Is a Key Player in Diphenic Caste Development

Avani Patel, M. Kim Fondrk, Osman Kaftanoglu, Christine Emore, Greg Hunt, Katy Frederick, Gro Amdam

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Background:Honey bees (Apis mellifera) provide a principal example of diphenic development. Excess feeding of female larvae results in queens (large reproductives). Moderate diet yields workers (small helpers). The signaling pathway that links provisioning to female developmental fate is not understood, yet we reasoned that it could include TOR (target of rapamycin), a nutrient- and energy-sensing kinase that controls organismal growth.Methodology/Principal Findings:Here, the role of Apis mellifera TOR (amTOR) in caste determination is examined by rapamycin/FK506 pharmacology and RNA interference (RNAi) gene knockdown. We show that in queen-destined larvae, the TOR inhibitor rapamycin induces the development of worker characters that are blocked by the antagonist FK506. Further, queen fate is associated with elevated activity of the Apis mellifera TOR encoding gene, amTOR, and amTOR gene knockdown blocks queen fate and results in individuals with worker morphology.Conclusions/Significance:A much-studied insect dimorphism, thereby, can be governed by the TOR pathway. Our results present the first evidence for a role of TOR in diphenic development, and suggest that adoption of this ancestral nutrient-sensing cascade is one evolutionary pathway for morphological caste differentiation in social insects.

Original languageEnglish (US)
Article numbere509
JournalPLoS One
Volume2
Issue number6
DOIs
StatePublished - 2007

Fingerprint

Sirolimus
queen insects
Apis mellifera
Social Class
Bees
caste determination
Apis mellifera mellifera
Gene Knockdown Techniques
genes
larvae
social insects
nutrients
Tacrolimus
pharmacology
dimorphism
RNA interference
Nutrients
honey bees
Larva
Insects

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Patel, A., Fondrk, M. K., Kaftanoglu, O., Emore, C., Hunt, G., Frederick, K., & Amdam, G. (2007). The Making of a Queen: TOR Pathway Is a Key Player in Diphenic Caste Development. PLoS One, 2(6), [e509]. https://doi.org/10.1371/journal.pone.0000509

The Making of a Queen : TOR Pathway Is a Key Player in Diphenic Caste Development. / Patel, Avani; Fondrk, M. Kim; Kaftanoglu, Osman; Emore, Christine; Hunt, Greg; Frederick, Katy; Amdam, Gro.

In: PLoS One, Vol. 2, No. 6, e509, 2007.

Research output: Contribution to journalArticle

Patel, A, Fondrk, MK, Kaftanoglu, O, Emore, C, Hunt, G, Frederick, K & Amdam, G 2007, 'The Making of a Queen: TOR Pathway Is a Key Player in Diphenic Caste Development', PLoS One, vol. 2, no. 6, e509. https://doi.org/10.1371/journal.pone.0000509
Patel A, Fondrk MK, Kaftanoglu O, Emore C, Hunt G, Frederick K et al. The Making of a Queen: TOR Pathway Is a Key Player in Diphenic Caste Development. PLoS One. 2007;2(6). e509. https://doi.org/10.1371/journal.pone.0000509
Patel, Avani ; Fondrk, M. Kim ; Kaftanoglu, Osman ; Emore, Christine ; Hunt, Greg ; Frederick, Katy ; Amdam, Gro. / The Making of a Queen : TOR Pathway Is a Key Player in Diphenic Caste Development. In: PLoS One. 2007 ; Vol. 2, No. 6.
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