With depleting natural resources and increasing material costs, the reusing of defective Roofing Shingles (RAS) has gained increasing attention. However, it has been noted that the use of aged asphalt materials can adversely affect low temperature performance shown both in the field through decreased pavement life and in the laboratory through the induction of micro-cracking. Another development in sustainable infrastructure is that of Bio-Modified Binder (BMB) particularly that derived from swine manure. The use of BMB in HMA has shown trends of increasing low temperature performance specifically in samples containing aged materials. In this paper, samples were subjected to dry air conditioning times of 0, 24, 48, and 72 hours to simulate low temperature field conditions. Through Bending Beam Rheometer (BBR) testing, stiffness showed a trend of being lower and m-values were higher in samples containing BMB in conditioned samples. Direct Tension Tester (DTT) results revealed congruent results; however, a drop in failure stress, strain, peak load, fracture energy, and ductility at 24 hour conditioning was observed to indicate micro-cracking as a discontinuity in results was observed.