The landscape of transcription errors in eukaryotic cells

Jean Francois Gout, Weiyi Li, Clark Fritsch, Annie Li, Suraiya Haroon, Larry Singh, Ding Hua, Hossein Fazelinia, Zach Smith, Steven Seeholzer, Kelley Thomas, Michael Lynch, Marc Vermulst

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Accurate transcription is required for the faithful expression of genetic information. To understand the molecular mechanisms that control the fidelity of transcription, we used novel sequencing technology to provide the first comprehensive analysis of the fidelity of transcription in eukaryotic cells. Our results demonstrate that transcription errors can occur in any gene, at any location, and affect every aspect of protein structure and function. In addition, we show that multiple proteins safeguard the fidelity of transcription and provide evidence suggesting that errors that evade these layers of RNA quality control profoundly affect the physiology of living cells. Together, these observations demonstrate that there is an inherent limit to the faithful expression of the genome and suggest that the impact of mutagenesis on cellular health and fitness is substantially greater than currently appreciated.

Original languageEnglish (US)
Article numbere1701484
JournalScience advances
Volume3
Issue number10
DOIs
StatePublished - Oct 1 2017
Externally publishedYes

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Eukaryotic Cells
Cell Physiological Phenomena
Mutagenesis
Quality Control
Proteins
Genome
RNA
Technology
Health
Genes

ASJC Scopus subject areas

  • General

Cite this

Gout, J. F., Li, W., Fritsch, C., Li, A., Haroon, S., Singh, L., ... Vermulst, M. (2017). The landscape of transcription errors in eukaryotic cells. Science advances, 3(10), [e1701484]. https://doi.org/10.1126/sciadv.1701484

The landscape of transcription errors in eukaryotic cells. / Gout, Jean Francois; Li, Weiyi; Fritsch, Clark; Li, Annie; Haroon, Suraiya; Singh, Larry; Hua, Ding; Fazelinia, Hossein; Smith, Zach; Seeholzer, Steven; Thomas, Kelley; Lynch, Michael; Vermulst, Marc.

In: Science advances, Vol. 3, No. 10, e1701484, 01.10.2017.

Research output: Contribution to journalArticle

Gout, JF, Li, W, Fritsch, C, Li, A, Haroon, S, Singh, L, Hua, D, Fazelinia, H, Smith, Z, Seeholzer, S, Thomas, K, Lynch, M & Vermulst, M 2017, 'The landscape of transcription errors in eukaryotic cells', Science advances, vol. 3, no. 10, e1701484. https://doi.org/10.1126/sciadv.1701484
Gout JF, Li W, Fritsch C, Li A, Haroon S, Singh L et al. The landscape of transcription errors in eukaryotic cells. Science advances. 2017 Oct 1;3(10). e1701484. https://doi.org/10.1126/sciadv.1701484
Gout, Jean Francois ; Li, Weiyi ; Fritsch, Clark ; Li, Annie ; Haroon, Suraiya ; Singh, Larry ; Hua, Ding ; Fazelinia, Hossein ; Smith, Zach ; Seeholzer, Steven ; Thomas, Kelley ; Lynch, Michael ; Vermulst, Marc. / The landscape of transcription errors in eukaryotic cells. In: Science advances. 2017 ; Vol. 3, No. 10.
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