The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids

W. J. Burrows, D. J. Armstrong, F. Skoog, Sidney Hecht, J. T A Boyle, N. J. Leonard, J. Occolowitz

Research output: Contribution to journalArticle

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Abstract

Two nucleosides responsible for cytokinin activity of Escherichia coli transfer ribonucleic acids have been isolated and identified as 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-D-ribofuranosylpurine and 6-(3-methyl- 2-butenylamino)-9-β-D-ribofuranosylpurine. The structures of these compounds were assigned on the basis of their ultraviolet and mass spectra, which were identical with those of the corre-spending synthetic compounds. The ribosides were about equally active in the tobacco bioassay. They were detected at ca. 5 × 10-3μM and gave maximum responses at ca. 7 × 10-1 μM. The free base 6-(3-methyl-2-butenylamino)-2-methylthiopurine was detected at ca. 2 × 10-3 μM as compared with ca. 1 × 10-4 μM as an average value for 6-(3-methyl-2-butenylamino)purine.

Original languageEnglish (US)
Pages (from-to)3071-3076
Number of pages6
JournalBiochemistry
Volume8
Issue number7
StatePublished - 1969
Externally publishedYes

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Cytokinins
Tobacco
Bioassay
Transfer RNA
Nucleosides
Biological Assay
Escherichia coli
N(6)-(delta(2)-isopentenyl)adenine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Burrows, W. J., Armstrong, D. J., Skoog, F., Hecht, S., Boyle, J. T. A., Leonard, N. J., & Occolowitz, J. (1969). The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids. Biochemistry, 8(7), 3071-3076.

The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids. / Burrows, W. J.; Armstrong, D. J.; Skoog, F.; Hecht, Sidney; Boyle, J. T A; Leonard, N. J.; Occolowitz, J.

In: Biochemistry, Vol. 8, No. 7, 1969, p. 3071-3076.

Research output: Contribution to journalArticle

Burrows, WJ, Armstrong, DJ, Skoog, F, Hecht, S, Boyle, JTA, Leonard, NJ & Occolowitz, J 1969, 'The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids', Biochemistry, vol. 8, no. 7, pp. 3071-3076.
Burrows WJ, Armstrong DJ, Skoog F, Hecht S, Boyle JTA, Leonard NJ et al. The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids. Biochemistry. 1969;8(7):3071-3076.
Burrows, W. J. ; Armstrong, D. J. ; Skoog, F. ; Hecht, Sidney ; Boyle, J. T A ; Leonard, N. J. ; Occolowitz, J. / The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids. In: Biochemistry. 1969 ; Vol. 8, No. 7. pp. 3071-3076.
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